PMID: 7537314Apr 12, 1995Paper

Hydrocoating: a new method for coupling biomolecules to solid phases

Journal of Immunological Methods
K GregoriusH I Elsner

Abstract

Solid-phase immunoassays such as enzyme-linked immunosorbent assays require one of the assay components to be immobilized. Most frequently this is achieved by passive adsorption of the antigen or antibody to a hydrophobic polymer surface composed of, e.g., polystyrene. Alternatively the biomolecule can be bound indirectly via passively adsorbed carrier proteins or directly via functional groups on the solid phase using cross-linking agents. Here we describe a new technique--hydrocoating--for covalent immobilization of biomolecules, such as peptides, in highly hydrophilic surroundings. Peptides were immobilized on microtiter plates via covalent bonds to an activated hydrophilic polymer. Soluble dextran was activated using 2,2,2-triflouroethanesulphonyl chloride (tresyl chloride) leading to activation of hydroxyl groups on the dextran polymer. This activated dextran molecule was immobilized on a surface containing amino groups leaving a sufficient number of active groups for secondary binding of other biomolecules. Peptides, that were either undetectable or poorly recognized when adsorbed on polystyrene, were readily recognized when immobilized by the hydrocoating technique. Furthermore, peptides immobilized by this method were r...Continue Reading

References

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Citations

Apr 1, 1999·Journal of Peptide Science : an Official Publication of the European Peptide Society·K GregoriusH I Elsner
Apr 5, 2001·Biosensors & Bioelectronics·A M SmithM E Meyerhoff
Oct 16, 2003·Biosensors & Bioelectronics·Julia YakovlevaJenny Emnéus
Feb 1, 1996·Current Opinion in Biotechnology·C H Self, D B Cook
May 5, 2007·Assay and Drug Development Technologies·Hiroko TogamePeter Reinemer
Dec 23, 2011·Journal of Biomedicine & Biotechnology·Brandon J Biesiadecki, J-P Jin
Sep 4, 2009·Applied Biochemistry and Biotechnology·Mario Ribeiro de Melo-JúniorLuiz Bezerra de Carvalho
Jun 11, 2002·Biochemical and Biophysical Research Communications·Soichi TanabeToshihide Nishimura
Jan 18, 2008·Journal of Clinical Laboratory Analysis·Zhenru FengBaohuan Sui
Feb 8, 2003·Chemistry & Biology·Mizuki TakahashiHisakazu Mihara
Aug 29, 2003·Biophysical Journal·Frank VollmerAlbert Libchaber
Jun 15, 1997·Analytical Chemistry·D J AndersonK A Davis

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