PMID: 9641843Jan 1, 1997Paper

Hydrocortisone Acetate Reference Standard (Control 961) of National Institute of Health Sciences

Kokuritsu Iyakuhin Shokuhin Eisei Kenkyūjo hōkoku = Bulletin of National Institute of Health Sciences
Y TagashiraS Okada

Abstract

The raw material of hydrocortisone acetate was tested for the preparation of the "Hydrocortisone Acetate Reference Standard (Control 961)". Analytical data obtained were as follows: IR spectrum, specific absorption wave numbers at 3428, 1748, 1723, 1631, and 1375 cm-1; specific absorbance, E1cm1% (242 nm) = 406; thin-layer chromatography, no impurities were detected until 0.05 microgram; high-performance liquid chromatography (HPLC), 2-3 impurities were detected and the amount of the total impurities was estimated to be about 0.2%; assay by HPLC, 100.7%. Based on the above results, the raw material was authorized as the Japanese Pharmacopoeia Reference Standard (Control 961).

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