Hydrocortisone enhances the barrier properties of HBMEC/ciβ, a brain microvascular endothelial cell line, through mesenchymal-to-endothelial transition-like effects

Fluids and Barriers of the CNS
Tomomi FurihataKan Chiba

Abstract

Because in vitro blood-brain barrier (BBB) models are important tools for studying brain diseases and drug development, we recently established a new line of conditionally immortalized human brain microvascular endothelial cells (HBMEC/ciβ) for use in such models. Since one of the most important functional features of the BBB is its strong intercellular adhesion, in this study, we aimed at improving HBMEC/ciβ barrier properties by means of culture media modifications, thus enhancing their use for future BBB studies. In addition, we simultaneously attempted to obtain insights on related mechanistic properties. Several types of culture media were prepared in an effort to identify the medium most suitable for culturing HBMEC/ciβ. The barrier properties of HBMEC/ciβ were examined by determining Na(+)-fluorescein permeability and transendothelial electric resistance (TEER). Endothelial marker mRNA expression levels were determined by quantitative real-time polymerase chain reaction. Adherens junction (AJ) formation was examined by immunocytochemistry. Cell migration ability was analyzed by scratch assay. Furthermore, cellular lipid composition was examined by liquid chromatography-time-of-flight mass spectrometry. Our initial screen...Continue Reading

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Citations

May 5, 2016·Stem Cells International·Damian Medici
Apr 19, 2016·Brain Research·Nurul Adhwa RahmanSiti Hanna Muharram
Apr 18, 2018·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Nick MernaSina Y Rabbany
May 30, 2020·Experimental & Molecular Medicine·Kyu Jin ChoiYoon-Jin Lee
Jun 26, 2020·Frontiers in Cellular Neuroscience·Brock R PluimerZhen Zhao
Mar 2, 2018·Biological & Pharmaceutical Bulletin·Meiyan ZhuTomomi Furihata
Jun 21, 2020·International Journal of Molecular Sciences·Daisy Y ShuMagali Saint-Geniez
Dec 20, 2018·Journal of Applied Toxicology : JAT·Kosuke SaitoKoichiro Tatsumi
Dec 12, 2018·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Kaoru Sato
Jan 13, 2021·International Journal of Molecular Sciences·Aleksandra MajewskaClaudine Kieda

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
RAP
PCR

Software Mentioned

Motic Image Plus
Statcell
2DICAL

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