PMID: 9449247Aug 1, 1997Paper

Hydrocortisone induces an increase of amylase content in individual zymogen granules from rat pancreas

The Journal of Steroid Biochemistry and Molecular Biology
M A MansoI De Dios

Abstract

This study was performed to evaluate the effects of different doses of hydrocortisone (1, 10 and 25 mg/kg/day) administered for 1, 3 and 8 days on pancreatic enzyme storage in rats. The enzyme content in both pancreas homogenates and in individual isolated zymogen granules (ZGs) was measured using standard biochemical assays and flow cytometry, respectively. Hydrocortisone did not alter the total amount of pancreatic DNA but increased the pancreas enzyme content in a time-dose-dependent way. Amylase activity was significantly increased after hydrocortisone administration at day +8 when 10 mg/kg/day was used, and from the first day of treatment when 25 mg/kg/day was administered. A significant increase in trypsin activity was also observed in response to 25 mg/kg/day of hydrocortisone but only from the third day of treatment onwards. As compared with control rats, chronic administration of either 1 or 10 mg/kg/day of hydrocortisone did not alter significantly either the size or the percentage of the two ZG subpopulations (Z1 and Z2) identified in the pancreas by flow cytometry; in addition, no significant changes were observed in the mean amylase content per individual granule, although its mean concentration increased in rats t...Continue Reading

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Citations

Dec 24, 1997·Molecular and Cellular Endocrinology·A C García-MonteroI de Dios
Jun 3, 1999·European Journal of Clinical Investigation·A C García-MonteroI De Dios
Dec 19, 2000·Biochimica Et Biophysica Acta·I de DiosM A Manso

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