Hydrocortisone regulation of hyaluronan metabolism in human skin organ culture

Journal of Cellular Physiology
U M AgrenR Tammi

Abstract

We studied the influence of hydrocortisone (HC) on hyaluronan (HA) metabolism in explants of human skin, a model retaining normal three-dimensional architecture of dermal connective tissue and dynamic growth and stratification of epidermal keratinocytes. The synthesis of hyaluronan and proteoglycans (PGs), and DNA, were determined with 3H-glucosamine and 3H-thymidine labelings, respectively. The total content and histological distribution of hyaluronan was studied utilizing a biotinylated aggrecan-link protein complex. A low concentration of HC (10(-9) M) stimulated the incorporation of 3H-glucosamine into hyaluronan in epidermis by 23% and reduced the disappearance rate of hyaluronan by 25% in chase experiments, resulting in a 74% increase in total hyaluronan (per epidermal dry weight) after a 5-day culture in 10(-9) M HC. On the other hand, a high concentration of HC (10(-5) M) reduced both synthesis (-42%) and degradation (-46%) of epidermal hyaluronan during 24 h labeling and chase periods. The cumulative effect of a 5-day treatment was a 24% decrease of total epidermal hyaluronan. The high dose (10(-5) M) also reduced keratinocyte DNA synthesis and epidermal thickness. In dermis, only the high (10(-5) M) concentration of H...Continue Reading

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Citations

May 20, 2004·Experimental Cell Research·Alberto PassiEdward V Maytin
Dec 18, 2007·Acta Physiologica·L RügheimerP Hansell
Dec 2, 2004·Archives of Histology and Cytology·Tadashi YasuiWilfried Meyer
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Dec 1, 2006·Micron : the International Research and Review Journal for Microscopy·Rodrigo Alves AzevedoLycia de Brito-Gitirana

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