Hydrogel-based microfluidic incubator for microorganism cultivation and analyses

Biomicrofluidics
Dietmar Puchberger-EnenglMichael J Vellekoop

Abstract

This work presents an array of microfluidic chambers for on-chip culturing of microorganisms in static and continuous shear-free operation modes. The unique design comprises an in-situ polymerized hydrogel that forms gas and reagent permeable culture wells in a glass chip. Utilizing a hydrophilic substrate increases usability by autonomous capillary priming. The thin gel barrier enables efficient oxygen supply and facilitates on-chip analysis by chemical access through the gel without introducing a disturbing flow to the culture. Trapping the suspended microorganisms inside a gel well allows for a much simpler fabrication than in conventional trapping devices as the minimal feature size does not depend on cell size. Nutrients and drugs are provided on-chip in the gel for a self-contained and user-friendly handling. Rapid antibiotic testing in static cultures with strains of Enterococcus faecalis and Escherichia coli is presented. Cell seeding and diffusive medium supply is provided by phaseguide technology, enabling simple operation of continuous culturing with a great flexibility. Cells of Saccharomyces cerevisiae are utilized as a model to demonstrate continuous on-chip culturing.

References

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Dec 31, 2011·Biomicrofluidics·Dietmar Puchberger-EnenglGerald A Urban
Feb 10, 2012·Lab on a Chip·Erwin BerthierDavid Beebe
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May 7, 2013·Lab on a Chip·Anja van de Stolpe, Jaap den Toonder
Jul 28, 2013·Lab on a Chip·Sebastiaan J TrietschPaul Vulto
Nov 5, 2013·Lab on a Chip·Frédérique DeissRatmir Derda
Nov 26, 2013·Lab on a Chip·Dietmar Puchberger-EnenglMichael J Vellekoop
Nov 26, 2013·Biomicrofluidics·Masoomeh TehranirokhJagat R Kanwar
Nov 8, 2014·Biomicrofluidics·Y C WeiJ Chen

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Citations

Apr 19, 2016·Lab on a Chip·Xuanqi ZhangChunxiong Luo
Sep 1, 2016·Biotechnology Progress·Aline F OliveiraLucimara G de la Torre
Nov 1, 2016·Biomedical Microdevices·Jennifer CampbellAlexis F Sauer-Budge
Jul 26, 2018·Frontiers in Microbiology·Marta Veses-GarciaAgneta Richter-Dahlfors
Aug 7, 2017·Technology·Fatemeh JalaliDaniel Irimia

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