Hydrogen Bond Surrogate Stabilization of β-Hairpins

ACS Chemical Biology
Nicholas Sawyer, Paramjit S Arora

Abstract

Peptide secondary and tertiary structure motifs frequently serve as inspiration for the development of protein-protein interaction (PPI) inhibitors. While a wide variety of strategies have been used to stabilize or imitate α-helices, similar strategies for β-sheet stabilization are more limited. Synthetic scaffolds that stabilize reverse turns and cross-strand interactions have provided important insights into β-sheet stability and folding. However, these templates occupy regions of the β-sheet that might impact the β-sheet's ability to bind at a PPI interface. Here, we present the hydrogen bond surrogate (HBS) approach for stabilization of β-hairpin peptides. The HBS linkage replaces a cross-strand hydrogen bond with a covalent linkage, conferring significant conformational and proteolytic resistance. Importantly, this approach introduces the stabilizing linkage in the buried β-sheet interior, retains all side chains for further functionalization, and allows efficient solid-phase macrocyclization. We anticipate that HBS stabilization of PPI β-sheets will enhance the development of β-sheet PPI inhibitors and expand the repertoire of druggable PPIs.

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Citations

Apr 8, 2020·Chemical Society Reviews·Elena Lenci, Andrea Trabocchi
Feb 13, 2021·Peptide Science·Haley I MerrittParamjit S Arora
Mar 23, 2021·Chembiochem : a European Journal of Chemical Biology·Sravanthi S ReddyErode N Prabhakaran
Jul 22, 2021·ACS Chemical Biology·Sarah L BlosserParamjit S Arora
Aug 14, 2020·Journal of the American Chemical Society·Daniel Y YooParamjit S Arora
Sep 10, 2021·The Journal of Organic Chemistry·Alexis D RichaudStéphane P Roche
Sep 14, 2021·Journal of the American Chemical Society·Jennifer R PaceJoshua A Kritzer

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