PMID: 7028115Aug 20, 1981Paper

Hydrogen bonding requirements for the insulin-sensitive sugar transport system of rat adipocytes

Biochimica Et Biophysica Acta
W D Rees, G D Holman

Abstract

(1) The t 1/2 for 1.3 mM D-allose uptake and efflux in insulin-stimulated adipocytes is 1.7 +/- 0.1 min. In the absence of insulin mediated uptake of D-allose is virtually eliminated and the uptake rate (t 1/2 = 75.8 +/- 4.99 min) is near that calculated for nonmediated transport. The kinetic parameters for D-allose zero-trans uptake in insulin-treated cells are Koizt = 271.3 +/- 34.2 mM, Voizt = 1.15 +/- 0.12 mM . s-1. (2) A kinetic analysis of the single-gate transporter (carrier) model interacting with two substrates (or substrate plus inhibitor) is presented. The analysis shows that the heteroexchange rates for two substrates interacting with the transporter are not unique and can be calculated from the kinetic parameters for each sugar acting alone with the transporter. This means that the equations for substrate analogue inhibition of the transport of a low affinity substrate such as D-allose can be simplified. It is shown that for the single gate transporter the Ki for a substrate analogue inhibitor should equal the equilibrium exchange Km for this analogue. (3) Analogues substituted at C-1 show a fused pyranose ring is accepted by the transporter. 1-Deoxy-D-glucose is transported but has low affinity for the transporter...Continue Reading

References

Mar 26, 1976·Molecular and Cellular Biochemistry·M P Czech
Jun 20, 1980·Biochimica Et Biophysica Acta·G D Holman
Jan 1, 1963·The Biochemical Journal·A Hernández, A Sols

❮ Previous
Next ❯

Citations

Aug 1, 1990·Journal of Bioenergetics and Biomembranes·P J Henderson
Jan 1, 1984·Progress in Biophysics and Molecular Biology·A Carruthers
Aug 1, 1997·Nuclear Medicine and Biology·F KoumanovD Fagret
Oct 24, 2006·Journal of Bacteriology·Marielena ChaviraWenyuan Shi
Aug 20, 2009·American Journal of Physiology. Endocrinology and Metabolism·Anthony CarruthersSherin U Devaskar
Apr 26, 2005·Bioscience, Biotechnology, and Biochemistry·Ghanwa AfachKen Izumori
Feb 25, 1999·Journal of Applied Microbiology·R J Lambert, M Stratford
Sep 8, 2000·Bioorganic & Medicinal Chemistry·A TatibouëtG D Holman
Mar 5, 1999·Journal of Molecular Biology·B N ChaudhuriS L Mowbray
Jan 1, 1986·FEBS Letters·S GameR Eisenthal
Nov 22, 2018·The Biochemical Journal·Geoffrey D Holman
Jan 30, 1990·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P J Henderson, M C Maiden
Jan 9, 2010·Rapid Communications in Mass Spectrometry : RCM·Lynsey N JonesAlison E Ashcroft
Jun 21, 2013·Organic & Biomolecular Chemistry·D Tyler McQuadePeter H Seeberger
Apr 1, 1986·The American Journal of Physiology·R L IngermannR H Bigley
Jun 22, 2017·Chembiochem : a European Journal of Chemical Biology·Marina Tanasova, Joseph R Fedie
Jul 16, 1999·Bioorganic & Medicinal Chemistry Letters·M Abbadi, C Morin
Sep 7, 1981·Biochimica Et Biophysica Acta·G D HolmanW D Rees
Feb 8, 1982·Biochimica Et Biophysica Acta·G D Holman, W D Rees
Dec 21, 1981·Biochimica Et Biophysica Acta·G D HolmanW D Rees
Jan 10, 1985·Biochimica Et Biophysica Acta·P J MidgleyG D Holman
Sep 1, 1987·Journal of Bacteriology·S A RobrishJ Thompson
Jan 24, 1998·Metabolism: Clinical and Experimental·M MatsuhisaY Yamasaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.