Hydrogen Concentration Profiles at the Oxic-Anoxic Interface: a Microsensor Study of the Hindgut of the Wood-Feeding Lower Termite Reticulitermes flavipes (Kollar).

Applied and Environmental Microbiology
A Ebert, Andreas Brune

Abstract

Molecular hydrogen is a key intermediate in lignocellulose degradation by the microbial community of termite hindguts. With polarographic, Clark-type H(inf2) microelectrodes, we determined H(inf2) concentrations at microscale resolution in the gut of the wood-feeding lower termite Reticulitermes flavipes (Kollar). Axial H(inf2) concentration profiles obtained from isolated intestinal tracts embedded in agarose Ringer solution clearly identified the voluminous hindgut paunch as the site of H(inf2) production. The latter was strictly coupled with both a low redox potential (E(infh) = -200 mV) and the absence of oxygen, in agreement with the growth requirements of the cellulolytic, H(inf2)-producing flagellates located in the hindgut paunch. Luminal H(inf2) partial pressures were much higher than expected (ca. 5 kPa) and increased more than threefold when the guts were incubated under a N(inf2) headspace. Radial H(inf2) concentration gradients showed a steep decrease from the gut center towards the periphery, indicating the presence of H(inf2)-consuming activities both within the lumen and at the gut epithelium. Measurements under controlled gas headspace showed that the gut wall was also a sink for externally supplied H(inf2), bo...Continue Reading

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Citations

Jan 13, 2015·Environmental Microbiology·Dörte HoffmannFerran Garcia-Pichel
Feb 4, 2014·Nature Reviews. Microbiology·Andreas Brune
Mar 10, 2004·Applied and Environmental Microbiology·Joseph R Graber, John A Breznak
Oct 6, 2012·Critical Reviews in Biotechnology·Anas Saadeddin
Mar 5, 2014·BMC Complementary and Alternative Medicine·Jinling XueYun Lu
Jan 29, 1999·Science·J R LeadbetterJ A Breznak
Apr 2, 2008·Annals of the New York Academy of Sciences·Yuchen Liu, William B Whitman
May 25, 2006·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Peter Stief, Gundula Eller
Feb 7, 2003·Applied and Environmental Microbiology·Hamadi I Boga, Andreas Brune
Jan 25, 2018·Yeast·Irene Stefanini
Aug 11, 2015·Frontiers in Microbiology·Michael NielsenMichael Kühl
Mar 25, 2020·International Journal of Environmental Research and Public Health·Hong LiuNing Ding
Jan 31, 2007·Applied and Environmental Microbiology·Michael PesterAndreas Brune
May 23, 2013·FEMS Microbiology Reviews·Philipp Engel, Nancy A Moran
Apr 7, 2005·Applied and Environmental Microbiology·Joseph R Graber, John A Breznak
May 19, 2005·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Martin Zimmer, Andreas Brune
Dec 8, 2015·Applied and Environmental Microbiology·Dorothee TegtmeierAndreas Brune
Oct 11, 2014·Environmental Science and Pollution Research International·Cédric TarayrePhilippe Thonart
Aug 1, 2013·Applied Microbiology and Biotechnology·Helmut KönigJürgen Fröhlich
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May 29, 2012·Applied and Environmental Microbiology·Nicholas R Ballor, Jared R Leadbetter
Dec 9, 2014·Frontiers in Microbiology·Rebeca B RosengausVeronica Godoy-Carter
Oct 2, 2007·Applied and Environmental Microbiology·Hisashi SatohSatoshi Okabe

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