Hydrogen-deuterium exchange mass spectrometry of membrane proteins in lipid nanodiscs

Chemistry and Physics of Lipids
Michelle RedhairWilliam M Atkins

Abstract

Hydrogen deuterium exchange mass spectrometry (H/DX MS) provides a quantitative comparison of the relative rates of exchange of amide protons for solvent deuterons. In turn, the rate of amide exchange depends on a complex combination of the stability of local secondary structure, solvent accessibility, and dynamics. H/DX MS has, therefore, been widely used to probe structure and function of soluble proteins, but its application to membrane proteins was limited previously to detergent solubilized samples. The large excess of lipids from model membranes, or from membrane fractions derived from in vivo samples, presents challenges with mass spectrometry. The lipid nanodisc platform, consisting of apolipoprotein A-derived membrane scaffold proteins, provides a native like membrane environment in which to capture analyte membrane proteins with a well defined, and low, ratio of lipid to protein. Membrane proteins in lipid nanodiscs are amenable to H/DX MS, and this is expected to lead to a rapid increase in the number of membrane proteins subjected to this analysis. Here we review the few literature examples of the application of H/DX MS to membrane proteins in nanodiscs. The incremental improvements in the experimental workflow of t...Continue Reading

Citations

Nov 14, 2019·Protein Science : a Publication of the Protein Society·Edgar A HodgeKelly K Lee
Jul 28, 2020·Biomedicines·Dominic NarangDerek J Wilson
Mar 15, 2020·Protein Science : a Publication of the Protein Society·Chloe Martens, Argyris Politis
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Feb 17, 2021·Communications Biology·Ramin Ekhteiari Salmas, Antoni James Borysik
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Jun 16, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Thirupathi RavulaAyyalusamy Ramamoorthy
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Jan 22, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Thirupathi RavulaAyyalusamy Ramamoorthy
Jan 26, 2022·Journal of the American Society for Mass Spectrometry·Didier DevaursAntoni J Borysik

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