PMID: 1204622Nov 15, 1975Paper

Hydrogen exchane at the beta-carbon of amino acids during transamination

European Journal of Biochemistry
U WalterH D Söling

Abstract

The hydrogen exchange at the Beta-carbon of L-alanine, L-glutamate and L-asparate with water has been examined during transamination catalyzed by glutamic-oxaloacetic transaminase and by glutamic-pyruvic transaminase. A significant hydrogen exchange at the Beta-carbon has been demonstrated during incubation of L-[3-3H]alanine + glutamic-pyruvic transaminase, L-[3-3H]alanine + alpha-oxo-glutarate + glutamic-pyruvic transaminase, L-[3-3H]glutamate + glutamic-oxaloacetic transaminase, L-[3-3H]glutamate + oxaloacetate +glutamic-oxaloacetic transaminase, and L-[3-3H]glutamate + pyruvate + glutamic-pyruvic transaminase as shown by the appearance of 3H2O. No hydrogen exchange at the Beta-carbon of L-glutamate occurred during incubation of L-[3-3H]-glutamate with glutamic-pyruvic transaminase alone. The hydrogen exchaned at the Beta-carbon of L-glutamate coincides with transamination as demonstrated by nuclear magnetic resonance studies of 2H2O-L-glutamate exchange during transamination by glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase. No hydrogen exchange at the Beta-carbon occurred during transamination of L-aspartate by glutamic-oxaloacetic transaminase as shown by nuclear magnetic resonance spectroscopy and co...Continue Reading

References

Feb 3, 1975·Biochemical and Biophysical Research Communications·P Marcotte, C Walsh
May 20, 1974·Biochemical and Biophysical Research Communications·U M Babu, R B Johnston
Jan 1, 1961·The Biochemical Journal·T OSHIMA, N TAMIYA
Mar 1, 1964·Biochemistry·C P HENSON, W W CLELAND

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Citations

Jan 15, 1980·Biochemical and Biophysical Research Communications·T A AlstonH J Bright
May 23, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Arthur J L CooperKarl Ploessl
Nov 15, 1977·FEBS Letters·F A Marston, C I Pogson
May 9, 2002·American Journal of Physiology. Cell Physiology·George A KimmichJoan Randles
May 1, 1980·European Journal of Biochemistry·P DiziolB M Babior
Jan 1, 1981·Pharmacology & Therapeutics·T A Alston
Apr 15, 1979·Biochemical Pharmacology·J KleinekeH D Söling
Jan 9, 1992·Biochimica Et Biophysica Acta·B Manuel y KeenoyW J Malaisse
Jul 1, 1988·Analytical Biochemistry·D GarrisonM A Namboodiri
Jan 30, 1977·Archives of Biochemistry and Biophysics·A GolichowskiW T Jenkins

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