Mar 25, 1976

Hydrogen exchange at the amide group of reduced pyridine nucleotides and the inhibition of that reaction by dehydrogenases

The Journal of Biological Chemistry
D G CrossH F Fisher

Abstract

Stopped flow ultraviolet spectroscopy has been used to measure the rate of hydrogen exchange with solvent at the amide group of reduced nicotinamide nucleotide coenzymes. Several mechanisms for the exchange reaction are considered in the light of the kinetic data. Complex formation between the coenzyme and any of four dehydrogenases markedly slows the rate of hydrogen exchange. Hydrogen bond formation and/or hydrophobic interactions within these complexes are thought to be the reasons for the decreased rate of exchange.

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Mentioned in this Paper

Ribonucleotides
Nicotinamide Assay
Pyridines
Amides
Alcohol Oxidoreductases
Nicotinamide Mononucleotide
Complex (molecular entity)
NADH
Plasma Protein Binding Capacity
Ultraviolet Spectroscopy

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