Hydrogen exchange in BPTI variants that do not share a common disulfide bond

Protein Science : a Publication of the Protein Society
B A Schulman, P S Kim

Abstract

Bovine pancreatic trypsin inhibitor (BPTI) is stabilized by 3 disulfide bonds, between cysteines 30-51, 5-55, and 14-38. To better understand the influence of disulfide bonds on local protein structure and dynamics, we have measured amide proton exchange rates in 2 folded variants of BPTI, [5-55]Ala and [30-51; 14-38]V5A55, which share no common disulfide bonds. These proteins resemble disulfide-bonded intermediates that accumulate in the BPTI folding pathway. Essentially the same amide hydrogens are protected from exchange in both of the BPTI variants studied here as in native BPTI, demonstrating that the variants adopt fully folded, native-like structures in solution. However, the most highly protected amide protons in each variant differ, and are contained within the sequences of previously studied peptide models of related BPTI folding intermediates containing either the 5-55 or the 30-51 disulfide bond.

References

Aug 28, 1975·Nature·C Chothia, J Janin
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J P Staley, P S Kim
Apr 3, 1992·Science·T E Creighton
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S Weissman, P S Kim
Nov 27, 1992·Cell·J S Weissman, P S Kim
Oct 15, 1991·Biochemistry·A D Robertson, R L Baldwin
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Jun 5, 1987·Journal of Molecular Biology·D J StatesM Karplus
Dec 5, 1987·Journal of Molecular Biology·A WlodawerC Woodward
Nov 3, 1988·Nature·T G Oas, P S Kim
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Jan 1, 1985·Methods in Enzymology·J S Richardson
Jan 18, 1972·Biochemistry·R S MoldayR G Kallen
Feb 14, 1971·Journal of Molecular Biology·B Lee, F M Richards
Oct 1, 1994·Protein Science : a Publication of the Protein Society·J P Staley, P S Kim
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J ClarkeA R Fersht
Oct 1, 1993·Protein Science : a Publication of the Protein Society·S F Betz

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Citations

Jul 1, 1997·Protein Science : a Publication of the Protein Society·J X Zhang, D P Goldenberg
Dec 1, 1995·Nature Structural Biology·J S Weissman, P S Kim
Jul 1, 2005·Protein Science : a Publication of the Protein Society·Xue LiJ Throck Watson

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