PMID: 2574Jan 1, 1976

Hydrogen ion concentration and oxygen uptake in an isolated canine hindlimb

Journal of Applied Physiology
A H Harken

Abstract

Oxygen utilization (VO2) and lactate production by an isolated perfused canine hindlimb was evaluated at various hydrogen ion concentrations. A membrane lung perfusion system was established such that blood flow and temperature could be fixed at normal levels. Oxygen, nitrogen, and carbon dioxide (CO2) gas flows to the membrane lung were independently regulated to provide a fixed arterial oxygen content (CaO2). By changing CO2 flow, the pH of the arterial blood was varied between 6.9 and 7.6 at 10-min intervals. The mean O2 delivery (CaO2 X blood flow) was between 16.3 ML O2/min and 20.5 ml O2/min. Standard error of the mean in each dog, however, was less than 0.4 ml O2/min. VO2 was linearly related to the pH of the perfusing blood: VO2% = 100.1 pH - 643 (r = 0.866). Oxygen consumption was inversely related to PCO2: VO2% = -0.62 PCO2 + 124, but the correlation was less good (r = 0.729). Lactate production was linearly related to the pH of the perfusing blood (above a pH of 7.4): lactate produced = 22.5 pH - 162.5 (r = 0.75). At a pH below 7.4, lactate was not produced. Oxygen consumption of skeletal muscle appears critically dependent on extracellular fluid pH. A change in pH of 0.1 alters VO2 almost exactly 10%. Alkalosis is a...Continue Reading

Citations

Jan 1, 1994·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·F BeyersdorfP Satter
Jun 1, 1994·Pflügers Archiv : European journal of physiology·J Chen, P D Gollnick
Oct 1, 1980·Pflügers Archiv : European journal of physiology·S Krausz, S F Sullivan
Jun 1, 1996·The British Journal of Surgery·K IhnkenU Unkelbach
Jan 1, 1986·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·P E Bickler
Jan 1, 1977·Annals of the New York Academy of Sciences·J A DempseyA D Claremont
Jan 1, 1983·European Journal of Applied Physiology and Occupational Physiology·R BulbulianR A Wiswell
Jan 1, 1990·Pflügers Archiv : European journal of physiology·P D GollnickD R Hodgson
Jan 1, 1977·Acta Anaesthesiologica Scandinavica·D B StokkeG Juel

Related Concepts

Blood Glucose
Carbon Dioxide
Canis familiaris
Hindlimb
Hydrogen-Ion Concentration
Lactates
Muscle
Oxygen Consumption
Pyruvates

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