PMID: 11315194Apr 21, 2001Paper

Hydrogen peroxide and hydroxyl radical involvement in the activation of caspase-3 in chemically induced apoptosis of HL-60 cells

Cellular and Molecular Life Sciences : CMLS
K KajiwaraS Kojo

Abstract

Apoptosis of HL-60 cells induced by actinomycin D, H7, or daunorubicin was shown to involve the activation of caspase-3-like protease, 2 h after the addition of these drugs, based on microassay of enzyme activity by high-performance liquid chromatography. Catalase and a spin trap, N-t-butyl-alpha-phenylnitrone, which effectively inhibited the apoptosis induced by these drugs, also inhibited the activation of caspase-3-like protease. These results suggest that hydrogen peroxide and the hydroxyl radical are common mediators of caspase-3 activation caused by these chemicals, with apparently different functional mechanisms. Based on mitochondrial activity determined by oxygen consumption, complexes I, II, and IV were inhibited by actinomycin D. H7 inhibited complexes I and IV, 1 and 1.5 h respectively, after the addition of the drug to HL-60 cells. Daunorubicin inhibited complex IV, 1.5 h after the addition of the drug to HL-60 cells. Inhibition of complex IV by actinomycin D, H7, and daunorubicin were almost fully restored by the addition of cytochrome c. The release to the cytosol of cytochrome c by these drugs was also demonstrated by Western blot analysis. Addition of catalase inhibited the depression of complex IV activity ind...Continue Reading

Citations

Feb 15, 2002·Bioorganic & Medicinal Chemistry Letters·Sakiko HarunaShosuke Kojo
Nov 30, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Marcelo Hermes-Lima, Tania Zenteno-Savín
May 25, 2002·The European Journal of Neuroscience·D VaudryB J Gonzalez
Jun 26, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Tarunpreet Singh ThindSaroj Arora
Apr 27, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Katarzyna KaniaZofia Jóźwiak
Feb 1, 2005·Life Sciences·Hideki MizutaniShosuke Kawanishi
Aug 2, 2002·Biochemical and Biophysical Research Communications·Jürgen BockErich Gulbins
Sep 29, 2004·Free Radical Biology & Medicine·Benedicta N NukunaVernon E Anderson
Jul 1, 2004·Bioorganic & Medicinal Chemistry Letters·Miho YamaguchiShosuke Kojo
Oct 25, 2003·Biochemical and Biophysical Research Communications·Toshiyuki NakamuraYasuhito Nakagawa
Nov 10, 2018·Electromagnetic Biology and Medicine·Timur SalievSergey Mikhalovsky
Jun 18, 2002·The Journal of Biological Chemistry·Hideki MizutaniShosuke Kawanishi
Jun 13, 2020·Nanomaterials·Surachet DuanghathaipornsukDong-Shik Kim
Jul 12, 2019·Cancer Research·Santanu BhattacharyaDebabrata Mukhopadhyay

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