Hydrogen peroxide-induced apoptosis in human gastric carcinoma MGC803 cells

Cell Biology International
Yubin MaoShideng Bao

Abstract

Hydrogen peroxide (H(2)O(2)), a representative ROS, has been used to study the apoptosis of cancer cells to oxidative stress. In this study, we exploited the cellular and molecular mechanisms involved in H(2)O(2)-induced apoptosis in human gastric carcinoma MGC803 cells. Exposure of cells to H(2)O(2) might cause significant viability loss and the increase in apoptotic rate. Treatment with 0.4 mmol/L H(2)O(2) up-regulated Bax but down-regulated Bcl-2 in a time-dependent manner, while Bcl-xL expression remained unchanged. Our results also showed that the levels of Fas and Fas-L were increased, the pro-caspase-3 and pro-caspase-9 were down-regulated in H(2)O(2)-treated MGC803 cells. Under H(2)O(2) stress, we found that the protein p53 also participated in MGC803 cells apoptosis. Taken together, the present study indicated that Fas-mediated cell surface death receptor pathway and mitochondria-mediated pathway may participate in regulating the MGC803 cells apoptosis under oxidative stress.

Citations

Jan 4, 2012·Clinical and Experimental Pharmacology & Physiology·Zhengguang WangBenli Jia
Dec 24, 2013·World Journal of Gastroenterology : WJG·Samantha Shi Min LingBow Ho
Feb 9, 2008·Digestive Diseases and Sciences·Anna M LeungThomas A Miller
Feb 7, 2012·Regulatory Toxicology and Pharmacology : RTP·R JubendradassP P Mathur
Apr 21, 2009·Toxicology and Applied Pharmacology·Mahadevan JanaRama Rajaram
Jul 16, 2008·Acta histochemica·Rosa María Vigueras-VillaseñorJulio César Rojas-Castañeda
Jul 18, 2008·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Seung-Ki MinEun-Cheol Kim
Oct 15, 2013·International Journal of Medical Microbiology : IJMM·Nadzeya KramkoHans-Joachim Schnittler
Aug 16, 2013·Oncology Letters·Yubin MaoHao Yin
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Celso AlvesRui Pedrosa

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