PMID: 9658308Jul 11, 1998Paper

Hydrogen peroxide-induced cellular injury is associated with increase in endogenous fluorescence from rat gastric mucosal epithelial cell culture: A new method for detecting oxidative cellular injury by fluorescence measurement

Journal of Gastroenterology
H MatsuiT Ohno

Abstract

To develop a new method of detecting cellular injury caused by oxygen radicals, we studied endogenous fluorescence from the cultured cells of a rat gastric mucosal epithelial cell line. Measurement with an ultra-high sensitivity camera-image processor system under an inverted epifluorescence microscope showed that the fluorescence intensity of the cells increased time- and dose-dependently after the addition of hydrogen peroxide (H2O2), an oxygen radical precursor, to the medium. This increase was inhibited by the presence of catalase. Phase-contrast and fluorescence microscopy revealed that the fluorescence was emitted from granular substances in the cytoplasm of the injured cells. The spectral pattern of excitation and emission indicated that the fluorescent substances were flavins. In cell-free experiments, glutathione reductase which has flavin adenine dinucleotide (FAD) at the active site, increased in fluorescence after incubation with H2O2 in the presence of reduced glutathione and glutathione peroxidase. These findings indicate that FAD in the cytoplasm of cells injured by H2O2 increased in endogenous fluorescence according to the extent of injury, and suggest that fluorescence measurement may be a simple method in cell...Continue Reading

Citations

Nov 22, 2012·Journal of Clinical Biochemistry and Nutrition·Yumiko NaganoIchinosuke Hyodo
Jul 27, 2011·Journal of Gastroenterology·Hirofumi MatsuiIchinosuke Hyodo
May 31, 2012·Digestive Diseases and Sciences·Hiromasa NakamizoToshifumi Hibi
Jul 31, 2007·Microscopy Research and Technique·Bartek RajwaJ P Robinson
Feb 2, 2019·Journal of Clinical Biochemistry and Nutrition·Go YasudaHirofumi Matsui

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