Hydrogen peroxide induces heat shock protein and proto-oncogene mRNA accumulation in Xenopus laevis A6 kidney epithelial cells

Canadian Journal of Physiology and Pharmacology
M MullerJ J Heikkila

Abstract

In this study, we examined the effect of hydrogen peroxide on the accumulation of various mRNAs encoding heat shock proteins (hsps) and proto-oncogenes in Xenopus A6 kidney epithelial cells. Hydrogen peroxide treatment enhanced the accumulation of hsp90, hsp70, hsp30, c-jun, c-fos, and actin mRNAs with distinct temporal patterns. Although hsp70, c-fos, and c-jun mRNA levels peaked at 1-2 h before declining, hsp30 and hsp90 mRNA levels were maximal at 4-6 h. Other mRNAs, including heat shock cognate hsc70, immunoglobulin binding protein, and ribosomal L8, were unaffected. Treatment of kidney cells with a combination of mild heat shock plus hydrogen peroxide resulted in a synergistic increase in the relative levels of both hsp70 and hsp30 mRNA, but not hsp90, c-fos, c-jun, or actin. This study suggests that analysis of hsp and proto-oncogene mRNA levels may be of value as molecular biomarkers of oxidative stress associated with various disease states and nephrotoxicity in kidney.

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Citations

Feb 9, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·John J Heikkila
Mar 2, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Shantel E Walcott, John J Heikkila
Apr 7, 2009·Biochemical and Biophysical Research Communications·Noriko FukushimaMasaru Ryoji
Oct 7, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jessica P Woolfson, John J Heikkila
Oct 28, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·John J Heikkila
Jun 1, 2006·Current Protocols in Toxicology·Linda L Agnew, Kenneth Watson

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