PMID: 2508753Oct 30, 1989Paper

Hydrogen peroxide insult in cultured mammalian cells: relationships between DNA single-strand breakage, poly(ADP-ribose) metabolism and cell killing

Biochimica Et Biophysica Acta
O CantoniD Murray

Abstract

We examined the effect of exposure to H2O2 at 37 degrees C on Chinese hamster ovary cell survival, DNA single-strand break (SSB) induction and rejoining, and activation of poly(ADP-ribose) (ADPR) polymerase. The effect of the ADPR polymerase inhibitor 3-aminobenzamide on each of these processes was also determined. SSB induction increased progressively with increasing H2O2 concentration. SSB levels were maximal after approx. 5 min of exposure to H2O2 (100 microM) and then decreased at longer times. This decrease, which paralleled the time-dependent depletion of H2O2, was due to the rejoining of SSBs. 3-Aminobenzamide enhanced the level of SSBs at each time point. H2O2 increased the level of both ADPR synthesis and NAD+ depletion (both measures of ADPR polymerase activity) in a concentration-dependent fashion, with the maximum effect being reached after approx. 20 min. After 100 microM H2O2, the effects on both ADPR and NAD+ were reversible. 3-Aminobenzamide completely blocked the effects of the oxidant on both NAD+ and ADPR levels. Thus, SSB induction by H2O2 at 37 degrees C was accompanied by a marked but reversible stimulation of ADPR polymerase. However, cell killing by H2O2 was only slightly enhanced in the presence of 3-am...Continue Reading

References

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Citations

May 26, 1993·Molecular and Cellular Biochemistry·D LautierG G Poirier
Jan 1, 1990·Chemico-biological Interactions·M FioraniO Cantoni
Feb 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J Davies, J K Chipman
Jan 1, 1991·Free Radical Biology & Medicine·M A Baker, S Q He
Jan 1, 1992·Free Radical Biology & Medicine·R W ByrnesD H Petering
Jul 1, 1994·Free Radical Biology & Medicine·J P Kehrer, L G Lund
Feb 1, 1994·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·O CantoniF Cattabeni
Feb 26, 2004·Biochimica Et Biophysica Acta·Zuo-Hui ShaoChun-Su Yuan
Dec 4, 2003·Free Radical Biology & Medicine·Daniela CaporossiMaria Giulia Farace
Jun 6, 2000·Mutation Research·J Termini
Nov 14, 1997·General Pharmacology·O Cantoni, P Giacomoni
Apr 1, 2006·American Journal of Respiratory Cell and Molecular Biology·George F BarkerAaron B Waxman
Jul 18, 2003·BMC Cell Biology·Georges NoëlVincent Favaudon
Dec 23, 2009·Free Radical Biology & Medicine·Betul CatalgolTilman Grune
Mar 13, 2001·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Z GaoH Xu
Jun 1, 1997·Free Radical Research·A GuidarelliO Cantoni
Jan 1, 1992·Free Radical Research Communications·S K Jonas, P A Riley
Nov 18, 2005·Bioorganic & Medicinal Chemistry Letters·Xiao-Ling CockcroftGraeme C M Smith
Oct 19, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jenny Arnold, Tilman Grune

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