Hydrogen peroxide is a common signal for darkness- and ABA-induced stomatal closure in Pisum sativum

Functional Plant Biology : FPB
Radhika DesikanSteven Neill

Abstract

The requirement for hydrogen peroxide (H2O2) generation and action during stomatal closure induced by darkness and abscisic acid (ABA) was investigated in pea (Pisum sativum L.). Stomatal closure induced by darkness or ABA was inhibited by the H2O2-scavenging enzyme catalase or the antioxidant N-acetyl cysteine (NAC), or by diphenylene iodonium (DPI), an inhibitor of the H2O2-generating enzyme NADPH oxidase. Exogenous H2O2 induced stomatal closure in a dose- and time-dependent manner, and H2O2 was also required for ABA-inhibition of stomatal opening in the light. H2O2 accumulation in guard cells was increased by darkness or ABA, as assessed with the fluorescent dye dichlorodihydrofluorescein diacetate (H2-DCFDA) and confocal microscopy. Such increases were inhibited by catalase, NAC or DPI, consistent with the effects of these compounds on stomatal apertures. Employing polymerase chain reaction (PCR) with degenerate oligonucleotide primers, several NADPH oxidase homologues were identified from pea genomic DNA that had substantial identity to the Arabidopsis thaliana (L.) Heynh. rboh (respiratory burst oxidase homologue) genes. Furthermore, an antibody raised against the tomato rboh identified immunoreactive proteins in epiderma...Continue Reading

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Citations

Nov 1, 2017·Photosynthesis Research·Ekaterina SukhovaVladimir Sukhov
Jul 1, 2005·Functional Plant Biology : FPB·Priscila L GratãoRicardo A Azevedo
Aug 1, 2008·Journal of Integrative Plant Biology·Li-Juan QuanHong-Yu Li
Dec 22, 2005·The Plant Journal : for Cell and Molecular Biology·Jo BrightSteven J Neill
Sep 12, 2006·The Plant Journal : for Cell and Molecular Biology·Radhika DesikanSteven J Neill
Dec 20, 2008·Journal of Integrative Plant Biology·Xiao-Ping She, Xi-Gui Song
Oct 2, 2013·Journal of Food Science and Technology·Ramkishan ChoudharyP L Swarnkar
Apr 10, 2013·International Journal of Molecular Sciences·Nyuk Ling MaSu Shiung Lam
Jul 1, 2007·Plant Signaling & Behavior·Gustavo E GudesblatPeter C Morris
May 9, 2006·Antioxidants & Redox Signaling·James R Stone, Suping Yang
Feb 12, 2013·The Plant Cell·Takahiro JoudoiSumio Iwai
Jun 9, 2021·The New Phytologist·Silvere Vialet-ChabrandTracy Lawson

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