Hydrogen peroxide-mediated necrosis induction in HUVECs is associated with an atypical pattern of caspase-3 cleavage

Experimental Cell Research
A CsordasD Bernhard

Abstract

Oxidative stress, continuously exerted during chronic inflammation, has been implicated as a major causative agent of cellular dysfunction and cell death. In the present study, we investigated the impact of oxidative stress on the mode of cell death in HUVECs using H2O2 as a model reagent. We found that the predominant form of cell death was necrosis. Necrosis induction was accompanied by a distinct mode of caspase-3 cleavage, yielding a 29-kDa fragment. While inhibition of caspases could not prevent the generation of the 29-kDa fragment, general protease inhibitors, such as leupeptin and LLNL, proved to be effective in inhibiting the distinct processing pattern of caspase-3. These results suggest that caspases can act as substrates for non-caspase proteases in cells primed for necrosis induction. Thus, the pattern of caspase-3 cleavage might reflect the proteolytic system engaged in the cell death machinery in HUVECs.

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Citations

Sep 25, 2012·Free Radical Research·Saptarshi KarMahendra Kavdia
Apr 15, 2008·Atherosclerosis·Blair HendersonGeorg Wick
Dec 5, 2006·Atherosclerosis·Astrid SchmiederDavid Bernhard
Sep 26, 2009·Journal of Cellular Physiology·Veronica BovenziFernand Gobeil
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Sep 10, 2011·Chemical Research in Toxicology·Simona SeriniGabriella Calviello
Jan 16, 2018·Oxidative Medicine and Cellular Longevity·Nikolay V GoncharovPavel V Avdonin
Jun 27, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Barbara MessnerDavid Bernhard
Aug 1, 2018·Journal of Tissue Engineering and Regenerative Medicine·Felipe MonteVenu G Varanasi
Jan 11, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tali TohamiMichael Lishner
Oct 2, 2021·Frontiers in Cell and Developmental Biology·Sindhushree RaghunandanShankar Subramaniam

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