PMID: 2657036Mar 1, 1989

Hydrogen production by rumen holotrich protozoa: effects of oxygen and implications for metabolic control by in situ conditions

The Journal of protozoology
D LloydA G Williams

Abstract

Experiments with washed suspensions of holotrich protozoa (Isotricha spp. and Dasytricha ruminantium) showed that both organisms have an efficient O2-scavenging capability (apparent Km values 2.3 and 0.3 microM, respectively). Reversible inhibition of H2 production increased almost linearly with increasing O2 up to 1.5 microM; higher levels of O2 gave irreversible inhibition. In situ determinations of H2, CH4, O2 and CO2 in ovine rumen liquor, using a membrane inlet mass spectrometer probe, indicated that O2 was present before feeding at 1-1.5 microM and decreased to undetectable levels (less than 0.25 microM) within 25 min after feeding. A transient increase in O2 concentration after feeding occurred only in defaunated animals and resulted in suppression of CH4 and CO2 production. The presence of washed holotrich protozoa decreases the O2 sensitivity of CH4 production by suspensions of a cultured methanogenic bacterium Methanosarcina barkeri. It is concluded that holotrich protozoa play a role in ruminal O2 utilization as well as in the production of fermentation end products (especially short-chain volatile fatty acids) utilized by the ruminant and H2 utilized by methanogenic bacteria. These hydrogenosome-containing protozoa ...Continue Reading

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Citations

Jun 14, 2002·International Journal for Parasitology·Kevin S W TanEu Hian Yap
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·E T BuiP J Johnson
Feb 22, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·T Martin EmbleyPeter Foster
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Sep 10, 2014·FEMS Microbiology Ecology·Alejandro BelancheCharles J Newbold
Jul 22, 2020·Nature Reviews. Microbiology·Guillaume BorrelChristine Moissl-Eichinger

Related Concepts

Hydrogen
Dioxygen
Rumen
Oreamnos americanus

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