Hydrogen sulfide attenuates epithelial-mesenchymal transition of human alveolar epithelial cells

Pharmacological Research : the Official Journal of the Italian Pharmacological Society
Liping FangXinmin Liu

Abstract

We previously reported that the endogenous cystathionine gamma-lyase (CSE)/hydrogen sulfide (H(2)S) pathway is implicated in the pathogenesis of bleomycin-induced pulmonary fibrosis in rats, but the exact cellular mechanisms are not well characterized. Epithelial-mesenchymal transition (EMT), induced by transforming growth factor beta1 (TGF-beta1) in alveolar epithelial cells, plays an important role in the pathogenesis of pulmonary fibrosis. We studied whether H(2)S could attenuate EMT in cultured alveolar epithelial cells and TGF-beta1 treatment suppressed CSE expression in A549 cells. Inhibition of endogenous CSE by dl-propargylglycine led to spontaneous EMT, as manifested by decreased E-cadherin level, increased vimentin expression and fibroblast-like morphologic features. Exogenous H(2)S applied to TGF-beta1-treated A549 cells decreased vimentin expression, increased E-cadherin level and retained epithelial morphologic features. In addition, preincubation with H(2)S decreased Smad2/3 phosphorylation in A549 cells stimulated by TGF-beta1, and H(2)S-inhibited alveolar EMT was mimicked by treatment with SB505124, a Smad2/3 inhibitor, but not pinacidil, an ATP-sensitive K(+) channel (K(ATP)) opener. H(2)S serves a critical rol...Continue Reading

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Citations

Apr 6, 2012·Respiratory Physiology & Neurobiology·Yahong Chen, Rui Wang
Jun 17, 2015·Oxidative Medicine and Cellular Longevity·Kai SongLi-Fang Hu
Jun 17, 2015·Oxidative Medicine and Cellular Longevity·Shufang ZhangGensheng Zhang
Feb 25, 2015·Chemical Science·J CaoA R Lippert
Mar 1, 2012·Journal of Breath Research·Nikolay IshkitievToshio Imai
Oct 23, 2020·Expert Review of Respiratory Medicine·Marta SchiliroChristina Pabelick
Jan 24, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Kelly AscençãoCsaba Szabo
Jun 1, 2021·Seminars in Cancer Biology·Hyun-Wook LeeSuresh Cuddapah
Nov 8, 2017·Chemical Reviews·Milos R FilipovicRuma Banerjee

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