Hydrogen sulfide exposure induces pyroptosis in the trachea of broilers via the regulatory effect of circRNA-17828/miR-6631-5p/DUSP6 crosstalk on ROS production.

Journal of Hazardous Materials
Nuan SongShu Li

Abstract

Hydrogen sulfide (H2S) is an air pollutant to cause tracheal injury. Pyroptosis is responsible for tissue injury through reactive oxygen species (ROS) production. Competitive endogenous RNAs (ceRNAs) chelate microRNAs and reduce their inhibitory effect on other transcripts, thus affecting ROS levels and pyroptosis. However, it is not clear how H2S regulates pyroptosis via the ceRNA axis. Therefore, we established a broilers model of H2S exposure for 42 days to assess pyroptosis and obtain a ceRNA network by immunohistochemistry and RNA sequencing. We detected pyroptosis induced by H2S and verified circRNA-IGLL1-17828/miR-6631-5p/DUSP6 axis by a double luciferase reporter assay. We also measured ROS levels and the expression of pyroptotic indicators such as (Caspase1) Casp-1, Interleukin 1β (IL-1β) and Interleukin 1β (IL-18). miR-6631-5p knockdown decreased pyroptotic indicators induced by H2S. Overexpression of miR-6631-5p or DUSP6 knockdown stimulated ROS generation and upregulated pyroptotic indicators. N-acetyl-L-cysteine (NAC) decreased pyroptotic indicators and ROS levels both induced by miR-6631-5p. Moreover, circRNA-IGLL1-17828, participated in intermolecular competition as a ceRNA of DUSP6. In conclusion, circRNA-IGLL1-...Continue Reading

References

May 31, 2008·Contributions to Microbiology·Balázs Rada, Thomas L Leto
Jan 17, 2009·Nature Reviews. Microbiology·Tessa BergsbakenBrad T Cookson
Mar 23, 2010·Cell·Kate Schroder, Jurg Tschopp
Mar 31, 2010·Amino Acids·Thomas P VacekSuresh C Tyagi
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Sep 26, 2013·Biochemical Society Transactions·Peter NichollsMike T Wilson
Jan 17, 2014·Nature·Yvonne TayPier Paolo Pandolfi
May 7, 2014·Chest·Guy G BrusselleMohamed Lamkanfi
May 27, 2014·Cell·Mohamed Lamkanfi, Vishva M Dixit
Oct 21, 2014·Antioxidants & Redox Signaling·Justine M AbaisPin-Lan Li
Apr 24, 2015·Journal of Agromedicine·Justene GuarrasiShelley Kirychuk
Oct 12, 2017·Nitric Oxide : Biology and Chemistry·Samantha L Malone RubrightJim Peterson
Sep 7, 2018·Military Medicine·Omar SaeedLeopoldo C Cancio
Nov 22, 2018·Frontiers in Immunology·Gustavo P Amarante-MendesKarina R Bortoluci
Mar 17, 2019·Cellular and Molecular Life Sciences : CMLS·Lu Liu, Bingwei Sun
Mar 30, 2019·Ecotoxicology and Environmental Safety·Dechun ChenXiaohua Teng
May 28, 2019·Cell Death and Differentiation·Peixing WanYehong Zhuo
Jun 20, 2019·Immunity·Nina Van Opdenbosch, Mohamed Lamkanfi
Aug 15, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Xiaona AnJianbo Wu
Oct 17, 2019·Investigative Ophthalmology & Visual Science·Chufeng GuQinghua Qiu
Oct 23, 2019·Environmental Science and Pollution Research International·Zheng LiZhanyong Wu
Dec 4, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Dong HeXing Wei
Feb 15, 2020·Current Opinion in Immunology·Longfei WangHao Wu
Apr 2, 2020·Journal of Experimental & Clinical Cancer Research : CR·Chengtao YuWenxin Qin

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Citations

Jul 5, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jianqing ChenShu Li
Jul 12, 2021·Ecotoxicology and Environmental Safety·Qianru ChiShu Li

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