Hydrogen sulfide induces hyperpolarization and decreases the exocytosis of secretory granules of rat GH3 pituitary tumor cells

Biochemical and Biophysical Research Communications
Alsu N MustafinaGuzel F Sitdikova

Abstract

The aim of the present study was to evaluate the effects of hydrogen sulfide (H2S) on the membrane potential, action potential discharge and exocytosis of secretory granules in neurosecretory pituitary tumor cells (GH3). The H2S donor - sodium hydrosulfide (NaHS) induced membrane hyperpolarization, followed by truncation of spontaneous electrical activity and decrease of the membrane resistance. The NaHS effect was dose-dependent with an EC50 of 152 μM (equals effective H2S of 16-19 μM). NaHS effects were not altered after inhibition of maxi conductance calcium-activated potassium (BK) channels by tetraethylammonium or paxilline, but were significantly reduced after inhibition or activation of ATP-dependent potassium channels (KATP) by glibenclamide or by diazoxide, respectively. In whole-cell recordings NaHS increased the amplitude of KATP currents, induced by hyperpolarizing pulses and subsequent application of glibenclamide decreased currents to control levels. Using the fluorescent dye FM 1-43 exocytosis of secretory granules was analyzed in basal and stimulated conditions (high K(+) external solution). Prior application of NaHS decreased the fluorescence of the cell membrane in both conditions which links with activation o...Continue Reading

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Citations

Jan 11, 2017·Antioxidants & Redox Signaling·Gopi K KolluruChristopher G Kevil
Jun 8, 2017·Cell Metabolism·Christopher HineJames R Mitchell
Jun 6, 2017·Bulletin of Experimental Biology and Medicine·D M GabitovaG F Sitdikova
Sep 29, 2020·Frontiers in Cellular Neuroscience·Kseniia KorolevaGuzel Sitdikova

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