Hydrogen sulfide prevents renal ischemia-reperfusion injury in CLAWN miniature swine

The Journal of Surgical Research
Mitsuhiro SekijimaKazuhiko Yamada

Abstract

Hydrogen sulfide (H2S) has recently been reported to demonstrate both antiinflammatory and cytoprotective effects; however, its efficacy has not been well documented in large animal models. In this study, we examined whether the administration of H2S offers cytoprotective effects on renal ischemia-reperfusion injury (IRI) in a preclinical miniature swine model. Major histocompatibility complex-inbred, CLAWN miniature swine (n = 9) underwent a right nephrectomy, followed by induction of a 120-min period of warm ischemia via placement of clamps on the left renal artery and vein. Group 1 (n = 3) underwent renal ischemia without H2S administration. Groups 2 (n = 3) and 3 (n = 3) received Na2S (prodrug of H2S) 10 min before reperfusion of the ischemic kidneys followed by a 30-min of Na2S postreperfusion intravenously (group 2) or selective administration of Na2S via the left renal artery (group 3). IRI was assessed by kidney biopsies, levels of inflammatory cytokines in sera and kidney tissue. Animals in group 1 had significantly higher serum creatinine levels compared with animals in groups 2 and 3 (P < 0.01). Histology showed severe tubular damage with TUNEL-positive cells in group 1 on postoperative day 2 compared with mild damag...Continue Reading

Citations

Nov 20, 2019·PloS One·Hanno MaassenAnnemieke M Coester
Dec 15, 2019·Archives of Physiology and Biochemistry·Rachid AkkiAngela Marino
Jul 21, 2020·American Journal of Physiology. Renal Physiology·Ilias P DoulamisJames D McCully
Jan 4, 2019·International Urology and Nephrology·Fikriye Yasemin OzatikZeynep Bayat
Aug 10, 2021·Molecular Medicine Reports·Hu ZhangNannan Guo
Nov 26, 2021·ANZ Journal of Surgery·Yi DengPaul N Smith

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