Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats

Molecular Medicine Reports
Lin LiJun Yang

Abstract

The present study aimed to explore the effect of hydrogen sulfide (H2S) on renal tissue fibrosis and its mechanism in diabetic rats. Rats were randomly divided into four groups (n=13/group): Control group; induced diabetes mellitus group (STZ); induced diabetes mellitus treated with H2S group (STZ + H2S); normal rats treated with H2S group (H2S). The diabetic model was induced by intraperitoneal (i.p.) injections of 40 mg/kg body weight streptozotocin (STZ); the control group was treated with saline every day (i.p); NaHS (100 µmol/kg i.p.) was administered to rats of STZ + H2S group and H2S group. After 8 weeks, rat body weight and 24 h proteinuria levels were determined in each group, renal pathological morphology was analyzed by Masson's trichrome staining, collagen IV content was detected by immunohistochemistry, and periodic acid‑Schiff (PAS) staining was performed on renal glomerular and tubular basement membranes. The expression levels of matrix metalloproteinase 9 (MMP9), MMP7, tissue inhibitor of metalloproteinase 1 (TIMP1), superoxide dismutase (SOD), serine/threonine kinase AKT, transforming growth factor (TGF)‑β1, nuclear factor (NF)‑κB and several autophagy related proteins were assessed by western blot analysis. Co...Continue Reading

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Citations

Nov 26, 2019·Chinese Medical Journal·Yu WangQiong-Jing Yuan
Apr 9, 2020·Diabetes, Obesity & Metabolism·Jonatan Barrera-Chimal, Frédéric Jaisser
Dec 20, 2019·Chinese Medical Journal·Yu WangQiong-Jing Yuan
Apr 23, 2019·Experimental and Therapeutic Medicine·Jianping LiuQinghong Cheng

Related Concepts

Autophagy
Western Blotting
Collagen Type IV
Diabetes
Diabetes Mellitus
Down-Regulation
Fibrosis
Hydrogen Sulfide
Immunohistochemistry
Kidney

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