Hydrogen sulfide upregulates miR-16-5p targeting PiK3R1 and RAF1 to inhibit neutrophil extracellular trap formation in chickens

Ecotoxicology and Environmental Safety
Kai YinHongjin Lin

Abstract

Hydrogen sulfide (H2S) is a toxic air pollutant that causes immune damage. Recent studies have found that neutrophil extracellular trap (NET) formation is one way in which neutrophils exert immune functions. In addition, the formation of NETs is also related to thrombosis and autoimmune diseases. Recent studies have shown that miRNAs are involved in the regulation of a variety of pathophysiological processes. Here, we investigated the role of H2S in regulating the formation of NETs by affecting miR-16-5p. Our study established an in vitro H2S exposure model for neutrophils using phorbol-myristate-acetate (PMA) to induce NET formation. We observed the morphological changes of cells with scanning electron microscopy and fluorescence microscopy. Then, the content of extracellular DNA and the expression of MPO and NE in each group were detected. The results showed that H2S inhibited the formation of NETs. The expression of miR-16-5p and its target genes PiK3R1 and RAF1 was then measured by qRT-PCR. H2S upregulated miR-16-5p and inhibited expression of the target genes PiK3R1 and RAF1, and it subsequently inhibited the Pi3K/AKT and ERK pathways and decreased respiratory burst levels. Furthermore, H2S attenuated inositol 1,4,5-trisph...Continue Reading

References

Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
Mar 22, 2006·Cellular Microbiology·Constantin F UrbanArturo Zychlinsky
Sep 21, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jeffry D SchroeterDavid C Dorman
Nov 28, 2006·Biochimica Et Biophysica Acta·James A McCubreyRichard A Franklin
Jan 11, 2007·The Journal of Cell Biology·Tobias A FuchsArturo Zychlinsky
May 23, 2007·Journal of Cellular Physiology·Prisco MirandolaMarco Vitale
Jul 17, 2007·Nature Reviews. Microbiology·Volker Brinkmann, Arturo Zychlinsky
Oct 17, 2008·Physiological Reviews·Susan F Steinberg
Aug 6, 2009·Annual Review of Genetics·Congcong He, Daniel J Klionsky
Jun 16, 2010·The Journal of Biological Chemistry·Annika VaarmannAndrey Y Abramov
Sep 11, 2010·International Immunopharmacology·V Kumar, A Sharma
Oct 27, 2010·The Journal of Cell Biology·Venizelos PapayannopoulosArturo Zychlinsky
Nov 10, 2010·Cell Research·Quinten RemijsenPeter Vandenabeele
Dec 21, 2010·Nature Chemical Biology·Abdul HakkimHerbert Waldmann
Apr 16, 2013·Antioxidants & Redox Signaling·David R Linden
Oct 16, 2014·Environmental Health and Preventive Medicine·Haider Abdul-Lateef Mousa
Jan 16, 2015·PLoS Pathogens·Tyler W R HalversonShawn Lewenza
Mar 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·David Nobuhiro DoudaNades Palaniyar
Sep 8, 2015·Cell Reports·Abhisek BhattacharyaN Tony Eissa
Oct 16, 2015·The Journal of Pathology·Bart CortjensReinout A Bem
Nov 4, 2015·OncoTargets and Therapy·Fangzheng WangFujun Hu
May 3, 2016·The Journal of Clinical Investigation·Ole E Sørensen, Niels Borregaard
Nov 2, 2016·Journal of Leukocyte Biology·Ava HosseinzadehConstantin F Urban
Jul 22, 2016·Annals of the New York Academy of Sciences·Wilson RumbeihaArthi Kanthasamy
Jan 25, 2017·Environmental Science and Pollution Research International·Xiaoyan JiaoXiaohua Teng
Jun 3, 2017·ELife·Elaine F KennyArturo Zychlinsky
Oct 11, 2017·Nature Reviews. Immunology·Venizelos Papayannopoulos
Feb 14, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Ana B ArroyoRocío González-Conejero

❮ Previous
Next ❯

Citations

Mar 7, 2021·International Journal of Molecular Sciences·Sonia ÁguilaConstantino Martínez
Mar 18, 2021·Journal of Hazardous Materials·Qi YuanZhengdong Zhang
Aug 12, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Nahzli DilekCsaba Szabo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Related Papers

Antioxidants & Redox Signaling
Benjamin Lee PredmoreGabriel Gojon
Acta Pharmacologica Sinica
Wei GuoYi-Zhun Zhu
Nitric Oxide : Biology and Chemistry
Rudolf WedmannMilos R Filipovic
American Journal of Physiology. Cell Physiology
Nancy L KanagyAndreas Papapetropoulos
© 2021 Meta ULC. All rights reserved