Hydrogen sulphide induces HIF-1α and Nrf2 in THP-1 macrophages

Biochimie
Lilian LohningerHilde Laggner

Abstract

The transcription factor HIF-1α regulates the adaptive response of cells to hypoxia and oxidative stress. In addition, an important regulatory role for HIF-1α in immune reactions and inflammation is suggested. The present study attempts to investigate the effect of the gaseous signalling molecule hydrogen sulphide (H2S) on HIF-1α in THP-1 macrophages using the slow H2S releasing donor GYY4137. We found that H2S induced HIF-1α protein accumulation in THP-1 macrophages in a concentration-dependent manner. Western blot analysis of cell fractions showed that HIF-1α protein translocates into the nucleus and leads to an increase of its target protein glucose transporter-1 (GLUT-1). Activation of nuclear factor-κB (NF-κB), as well as secretion of the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), were reduced in the presence of H2S. These findings indicate that HIF-1α accumulation due to H2S was not triggered by the NF-κB pathway. The antioxidant pathway Nrf2/HO-1 (nuclear factor erythroid 2-related factor 2/heme oxygenase-1) was activated by H2S. Inhibition of the p38 mitogen-activated protein kinase (MAPK) reversed H2S mediated effects, suggesting that the p38 MAPK pathway may be involved in H2S...Continue Reading

References

Jan 1, 1992·Free Radical Research Communications·R SchreckP A Baeuerle
Mar 16, 1995·Biochimica Et Biophysica Acta·J HanR J Ulevitch
Apr 1, 1993·Current Opinion in Cell Biology·J V Ruderman
Sep 29, 2004·Cellular Signalling·Christiane Brahimi-HornJacques Pouysségur
Apr 5, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Ramzi OckailiRakesh C Kukreja
Apr 21, 2009·Free Radical Biology & Medicine·Ling LiPhilip K Moore
Jul 18, 2009·Circulation Research·John W CalvertDavid J Lefer
Apr 13, 2010·Free Radical Research·Markus K MuellnerHilde Laggner
Feb 9, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·M Scott Vandiver, Solomon H Snyder
Jul 27, 2012·British Journal of Pharmacology·Bo WuRui Wang
Oct 30, 2012·Biochemical Pharmacology·Khosrow Kashfi, Kenneth R Olson
Jul 16, 2013·Acta Diabetologica·Eliana D'AraioAndrea Hodgkinson

❮ Previous
Next ❯

Citations

Nov 22, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lenka TomasovaMarcin Ufnal
Apr 12, 2017·Antioxidants & Redox Signaling·Shuai YuanChristopher G Kevil
Oct 4, 2017·Nature Reviews. Immunology·Cormac T Taylor, Sean P Colgan
Sep 1, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yu-Fu ZhouYa Ke
Aug 22, 2017·Pharmacological Reports : PR·Lenka TomasovaMarcin Ufnal
Feb 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kenneth R OlsonKarl D Straubg
Feb 23, 2020·Biomolecules·Ewelina ZaorskaMarcin Ufnal
Feb 3, 2021·European Journal of Pharmacology·Dan-Dan GuiZhi-Sheng Jiang
Sep 3, 2020·Immunology·Fei SunCong-Yi Wang
Aug 12, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Nahzli DilekCsaba Szabo
Apr 17, 2021·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Haixia WangJianjun Mu
Apr 27, 2021·Immunological Medicine·Ali Asghar KianiAmirhossein Nafari
Dec 19, 2016·Inorganic Chemistry·T Spencer BaileyMichael D Pluth

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis