Hydrogenases in Nostoc sp. Strain PCC 73102, a Strain Lacking a Bidirectional Enzyme.

Applied and Environmental Microbiology
P TamagniniP Lindblad

Abstract

The present study was carried out in order to examine and characterize the bidirectional hydrogenase in the cyanobacterium Nostoc sp. strain PCC 73102. Southern hybridizations with the probes Av1 and Av3 (hoxY and hoxH, bidirectional hydrogenase small and large subunits, respectively) revealed the occurrence of corresponding sequences in Anabaena variabilis (control), Anabaena sp. strain PCC 7120, and Nostoc muscorum but not in Nostoc sp. strain PCC 73102. As a control, hybridizations with the probe hup2 (hupL, uptake hydrogenase large subunit) demonstrated the presence of a corresponding gene in all the cyanobacteria tested, including Nostoc sp. strain PCC 73102. Moreover, with three different growth media, a bidirectional enzyme that was functional in vivo was observed in N. muscorum, Anabaena sp. strain PCC 7120, and A. variabilis, whereas Nostoc sp. strain PCC 73102 consistently lacked any detectable in vivo activity. Similar results were obtained when assaying for the presence of an enzyme that is functional in vitro. Native polyacrylamide gel electrophoresis followed by in situ hydrogenase activity staining was used to demonstrate the presence or absence of a functional enzyme. Again, bands corresponding to hydrogenase ac...Continue Reading

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Citations

Jun 7, 2011·Journal of Bacteriology·Paulo Oliveira, Peter Lindblad
Mar 15, 2014·PloS One·Elias EnglundPia Lindberg
Aug 29, 2001·FEMS Microbiology Reviews·P M VignaisJ Meyer
Aug 22, 2014·Environmental Microbiology·Paulo OliveiraPaula Tamagnini
Jan 5, 2002·Applied and Environmental Microbiology·Rikard Axelsson, Peter Lindblad
Oct 2, 2007·FEMS Microbiology Reviews·Paula TamagniniPeter Lindblad
Dec 15, 2015·Environmental Microbiology·Paulo OliveiraPaula Tamagnini
Apr 26, 2007·Journal of Industrial Microbiology & Biotechnology·Martin E Barrios-LlerenaPhillip C Wright
Feb 8, 2005·Current Microbiology·Melakeselam LeulAnita Sellstedt
Apr 19, 2013·Marine Drugs·A Catarina GuedesF Xavier Malcata
Oct 23, 2015·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Filipe PintoPaula Tamagnini
Jul 17, 2007·Applied and Environmental Microbiology·Johannes SjöholmPeter Lindblad
Dec 3, 2004·Applied and Environmental Microbiology·Gudrun BoisonHermann Bothe
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Paula TamagniniPeter Lindblad
Apr 30, 2008·BMC Microbiology·Asa AgervaldPeter Lindblad
Mar 17, 2009·BMC Microbiology·Marie HolmqvistPeter Lindblad

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