Attenuated total reflection Fourier transform infrared spectroscopy was used to probe the kinetics of hydrogen/deuterium exchange in Manduca sexta apolipophorin-III (apoLp-III). ApoLp-III is an exchangeable apolipoprotein that is made up of five elongated amphipathic alpha-helices in a helical bundle conformation in the monomeric lipid-free form. Upon interaction with phospholipids, it is postulated to undergo a large conformational change whereby the hydrophobic interior is exposed, facilitating binding to the lipid surfaces. We have used the lipid-free and dimyristoylphosphatidylcholine-bound apoLp-III to study the dynamically variable domains in the two forms. Three populations of amide protons varying in their hydrogen/deuterium exchange rates were found to exist: slow, intermediate, and fast exchanging, which could correspond to completely buried, partially buried, and solvent-exposed domains on the protein in both the states. In lipid-free apoLp-III, 36, 12, and 52% of the total residues contributed to the slow, intermediate, and fast exchanging populations, respectively. In the dimyristoylphosphatidylcholine-bound form, the corresponding distribution was 20, 16, and 64%, representing a 12% increase in the number of expos...Continue Reading
A water-lipid interface induces a highly dynamic folded state in apocytochrome c and cytochrome c, which may represent a common folding intermediate
Reversible unfolding of cytochrome c upon interaction with cardiolipin bilayers. 1. Evidence from deuterium NMR measurements
Fluctuations of protein structure as expressed in the distribution of hydrogen exchange rate constants
Alignment of the apolipophorin-III alpha-helices in complex with dimyristoylphosphatidylcholine. A unique spatial orientation.
Tertiary stability of native and methionine-80 modified cytochrome c detected by proton-deuterium exchange using on-line Fourier transform infrared spectroscopy
Determination of soluble and membrane protein structure by Fourier transform infrared spectroscopy. I. Assignments and model compounds
Structural and binding characteristics of the carboxyl terminal fragment of apolipophorin III from Manduca sexta
Investigation of secondary and tertiary structural changes of cytochrome c in complexes with anionic lipids using amide hydrogen exchange measurements: an FTIR study
Is the pre-Tg DSC endotherm observed with solid state proteins associated with the protein internal dynamics? Investigation of bovine serum albumin by solid state hydrogen/deuterium exchange
Lipid-triggered conformational switch of apolipophorin III helix bundle to an extended helix organization
Relationship of membrane sidedness to the effects of the lipophosphoglycan of Leishmania donovani on the fusion of influenza virus
Structural basis for the conformational adaptability of apolipophorin III, a helix-bundle exchangeable apolipoprotein
Protein secondary structure content in solution, films and tissues: redundancy and complementarity of the information content in circular dichroism, transmission and ATR FTIR spectra
An unusual soluble beta-turn-rich conformation of prion is involved in fibril formation and toxic to neuronal cells
Metal binding properties and structure of a type III metallothionein from the metal hyperaccumulator plant Noccaea caerulescens
Transmembrane helix stability: the effect of helix-helix interactions studied by Fourier transform infrared spectroscopy
Cholesterol modulates the exposure and orientation of pulmonary surfactant protein SP-C in model surfactant membranes
Evaluation of the information content in infrared spectra for protein secondary structure determination
Prediction of the functional class of lipid binding proteins from sequence-derived properties irrespective of sequence similarity.
Hydrogen-deuterium exchange in bovine serum albumin protein monitored by Fourier transform infrared spectroscopy, part II: kinetic studies
Structural characterization by computer experiments of the lipid-free LDL-receptor-binding domain of apolipoprotein E
Marine antimicrobial peptide arenicin adopts a monomeric twisted β-hairpin structure and forms low conductivity pores in zwitterionic lipid bilayers
Hydrogen-deuterium exchange in membrane proteins monitored by IR spectroscopy: a new tool to resolve protein structure and dynamics
Structural domain organization of gastric H+,K+-ATPase and its rearrangement during the catalytic cycle.
Structural dynamics of the Streptomyces lividans K+ channel (SKC1): secondary structure characterization from FTIR spectroscopy
Prediction of functional class of proteins and peptides irrespective of sequence homology by support vector machines
Multidrug resistance protein MRP1 reconstituted into lipid vesicles: secondary structure and nucleotide-induced tertiary structure changes
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