Hydrogenosomal succinyl-CoA synthetase from the rumen-dwelling fungus Neocallimastix patriciarum; an energy-producing enzyme of mitochondrial origin

Gene
Joel B DacksMark van der Giezen

Abstract

Hydrogenosomes are hydrogen-producing organelles that are related to mitochondria and found in a variety of evolutionarily unrelated anaerobic microbial eukaryotes. Similar to classic mitochondria, hydrogenosomes contain the enzyme catalyzing the only reaction of the citric acid cycle directly producing energy; succinyl-CoA synthetase. We have isolated and characterized the genes encoding both subunits of this enzyme from the anaerobic chytrid fungus Neocallimastix patriciarum, a model organism in hydrogenosome research. Both subunits contain all characteristic features of this enzyme, including predicted hydrogenosomal targeting signals. Phylogenetic analyses of succinyl-CoA synthetase clearly indicate its mitochondrial ancestry, both by affiliation with mitochondrially localized fungal homologues and by the sisterhood of the eukaryotic succinyl-CoA synthetase clade with alpha-proteobacteria. Our analyses of the Trichomonas vaginalis SCS sequences also confirmed the mitochondrial affiliation of these hydrogenosomal enzymes, in contrast to previous results. While both hydrogenosomal and mitochondrial succinyl-CoA synthetase homologues have been identified, no succinyl-CoA synthetase proteins were identifiable in taxa possessing...Continue Reading

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Citations

May 13, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Marek Mentel, William Martin
Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Miklós MüllerWilliam F Martin
Jun 17, 2009·The Journal of Eukaryotic Microbiology·Mark van der Giezen
May 3, 2008·Molecular Microbiology·Karleigh HamblinMark van der Giezen
Dec 10, 2013·Biochimie·Takashi Makiuchi, Tomoyoshi Nozaki

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