Hydrolysis of carbaryl by human serum albumin

Archives of Toxicology
Miguel A SogorbEugenio Vilanova

Abstract

Human serum (HS) and human serum albumin (HSA) were able to hydrolyse the carbamate carbaryl. Carbarylase activity found in HSA was slightly activated by 1 mM Zn2+, Mn2+, Cd2+, Ni2+ and Na+ and by 0.01 mM Pb2+. The organophosphorus compounds paraoxon and O-hexyl O-2,5-dichlorophenyl phosphoramidate, caprylic acid, palmitic acid and the carboxyl ester p-nitrophenyl butyrate inhibited the hydrolysis of carbaryl by HSA, being in the last case a competitive inhibition. Using selective amino acid reagents, we concluded that Cys, Trp, Arg and Tyr seem to play important roles in the carbarylase activity of HSA. In addition, Tyr and Arg seem to be located in the active centre of the enzyme since carbaryl protected the activity from the inhibition. It was concluded that HSA hydrolyses carbaryl by a mechanism similar to that described for rabbit serum albumin based in transient carbamylation of a Tyr residue. The extrapolation of the hydrolysis rate to physiological albumin concentrations suggests that albumin might be playing a critical role in the detoxication of carbaryl.

References

Nov 4, 1975·Biochemistry·G E Means, M L Bender
May 1, 1994·American Journal of Medical Genetics·C T Schrander-StumpelJ P Fryns
Apr 16, 2002·Chemical Research in Toxicology·Miguel A SogorbEugenio Vilanova
Dec 1, 1959·Biochemical Pharmacology·K B AUGUSTINSSON, J E CASIDA
Dec 1, 1959·Biochimica Et Biophysica Acta·J E CASIDA, K B AUGUSTINSSON

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Citations

Jun 30, 2009·Expert Opinion on Drug Delivery·D JereC S Cho
Dec 12, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Guang-Bo GeLing Yang
Nov 3, 2010·Toxicology Letters·Susanne MüllerGeorg Reiter
Mar 10, 2010·Chemico-biological Interactions·Miguel A Sogorb, Eugenio Vilanova
Jul 15, 2015·Bioorganicheskaia khimiia·N V GoncharovA I Ukolov
Jun 10, 2015·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Yoshihiko ShimokawaKen Umehara
Feb 10, 2007·Endocrinology·Carine De VrieseChristine Delporte
Jul 19, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nikolay V GoncharovPavel V Avdonin

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