Hydrolysis of non-cognate aminoacyl-adenylates by a class II aminoacyl-tRNA synthetase lacking an editing domain

FEBS Letters
Ita Gruic-SovuljIvana Weygand-Durasevic


Aminoacyl-tRNA synthetases, a group of enzymes catalyzing aminoacyl-tRNA formation, may possess inherent editing activity to clear mistakes arising through the selection of non-cognate amino acid. It is generally assumed that both editing substrates, non-cognate aminoacyl-adenylate and misacylated tRNA, are hydrolyzed at the same editing domain, distant from the active site. Here, we present the first example of an aminoacyl-tRNA synthetase (seryl-tRNA synthetase) that naturally lacks an editing domain, but possesses a hydrolytic activity toward non-cognate aminoacyl-adenylates. Our data reveal that tRNA-independent pre-transfer editing may proceed within the enzyme active site without shuttling the non-cognate aminoacyl-adenylate intermediate to the remote editing site.


Oct 25, 2011·Journal of the American Chemical Society·Jaya Sarkar, Susan A Martinis
Dec 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Srujana S YadavalliMichael Ibba
May 28, 2010·The Journal of Biological Chemistry·Anand Minajigi, Christopher S Francklyn
May 26, 2010·The Journal of Biological Chemistry·Morana DulicIta Gruic-Sovulj
Jul 10, 2012·The Journal of Biological Chemistry·Jiqiang LingMiljan Simonovic
Jul 22, 2011·Nucleic Acids Research·Xiaolong ZhouEn-Duo Wang
Apr 22, 2009·Annual Review of Microbiology·Jiqiang LingMichael Ibba
Nov 27, 2009·The Journal of Biological Chemistry·Min TanEn-Duo Wang
Oct 19, 2012·Biochemistry·John J Perona, Andrew Hadd
Nov 12, 2009·FEBS Letters·Rajat BanerjeeMichael Ibba
Apr 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ziwei LiuKarin Musier-Forsyth
Dec 12, 2012·Archives of Biochemistry and Biophysics·Jasmina Rokov-PlavecIvana Weygand-Durasevic
Aug 29, 2013·Science China. Life Sciences·Xiaolong Zhou, En-Duo Wang
Feb 5, 2008·Methods : a Companion to Methods in Enzymology·Kathryn E SplanSusan A Martinis
Nov 7, 2015·ACS Synthetic Biology·Joanne M L HoDieter Söll
Nov 19, 2011·Wiley Interdisciplinary Reviews. RNA·Hieronim Jakubowski
Jun 4, 2014·Chembiochem : a European Journal of Chemical Biology·Chenguang FanYane-Shih Wang
Jun 27, 2015·Nature Communications·Sadeem AhmadRajan Sankaranarayanan
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Dmitry TworowskiMark G Safro
Oct 8, 2016·Methods : a Companion to Methods in Enzymology·Nevena Cvetesic, Ita Gruic-Sovulj
Nov 13, 2018·Nucleic Acids Research·Lin ChenKarin Musier-Forsyth
Aug 25, 2017·Nature Microbiology·Kyle Mohler, Michael Ibba
Jun 18, 2014·The EMBO Journal·Nevena CvetesicIta Gruic-Sovulj
Jan 9, 2008·The Journal of Biological Chemistry·Kathryn E SplanKarin Musier-Forsyth
Apr 18, 2017·Nature Chemical Biology·Paola EstradaSatish K Nair
Dec 11, 2008·The Journal of Biological Chemistry·Bin ZhuEn-Duo Wang


Aug 1, 1972·The Biochemical Journal·R B Loftfield, D Vanderjagt
Nov 5, 1993·Journal of Molecular Biology·M FujinagaStephen Cusack
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·Penny J Beuning, Karin Musier-Forsyth
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Anthony C BishopPaul Schimmel
Oct 8, 2003·The Journal of Biological Chemistry·Fai-Chu WongKarin Musier-Forsyth
May 27, 2004·The Journal of Biological Chemistry·Min-Gang XuEn-Duo Wang
Apr 23, 2005·The Journal of Biological Chemistry·Ita Gruic-SovuljJohn J Perona
Apr 23, 2005·The Journal of Biological Chemistry·Nathan T UterJohn J Perona
Aug 2, 2005·FEBS Letters·Dragana AhelIvana Weygand-Durasevic
Jul 11, 2006·Journal of Molecular Biology·Ita Gruic-SovuljIvana Weygand-Durasevic
Dec 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jiqiang LingMichael Ibba

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