Hydrolysis of organophosphate insecticides by an immobilized-enzyme system

Biotechnology and Bioengineering
D M Munnecke


An enzyme preparation that could detoxify parathion and eight other organophosphate pesticides was covalently bound to either porous glass or porous silica beads. This immobilized-enzyme system was examined for its use in detoxification of pesticides in production wastewaters. The kinetics of parathion hydrolysis were examined at flow rates up to 96 liter/hr and at influent substrate concentrations ranging from 10--250 mg/liter. The enzyme reactor was able to hydrolyze 95% or more of the parathion added to industrial wastewaters generated during its production, thus reducing the effluent parathion concentration to below 500 ppb. Laboratory continuous-flow experiments were conducted for 70 days with industrial wastewater and indicated no loss in immobilized-enzyme activity. The influence of pH, temperature, solvents, and detergents on enzyme stability and activity and enzyme reactor kinetics will be discussed.


Aug 20, 1996·Biotechnology and Bioengineering·K E LeJeune, Alan J Russell
Nov 15, 2002·Biotechnology and Bioengineering·Mark ShimazuWilfred Chen
Nov 15, 1983·Experientia·L M Johnson, H W Talbot
Jan 1, 1996·Applied Biochemistry and Biotechnology·S C Hung, J C Liao
Nov 19, 2013·Journal of Inorganic Biochemistry·Lena J DaumannLawrence R Gahan
Mar 6, 1998·Trends in Biotechnology·W Chen, A Mulchandani
Feb 3, 1999·Annals of the New York Academy of Sciences·K E LeJeuneAlan J Russell
Jul 27, 1999·Chemico-biological Interactions·B D Di SioudiJ R Wild
Mar 3, 2005·Biosensors & Bioelectronics·Brandy J White, H James Harmon
Jul 26, 2005·Biochemical and Biophysical Research Communications·Hong LiuNing-Yi Zhou
Apr 22, 2003·Annual Review of Biomedical Engineering·Alan J RussellRichard R Koepsel
May 12, 2009·Advances in Applied Microbiology·Casey M TheriotAmy M Grunden
Dec 31, 2005·Journal of Bioscience and Bioengineering·Kanako TagoMasahito Hayatsu
May 27, 2011·Journal of Enzyme Inhibition and Medicinal Chemistry·K Y VasanthaA P Sattur


Jul 1, 1976·Applied and Environmental Microbiology·D M Munnecke
Jul 1, 1972·Biotechnology and Bioengineering·R D Mason, H H Weetall
Mar 1, 1977·Applied and Environmental Microbiology·D M Munnecke

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