Hydrolysis of organophosphate insecticides by an immobilized-enzyme system

Biotechnology and Bioengineering
D M Munnecke

Abstract

An enzyme preparation that could detoxify parathion and eight other organophosphate pesticides was covalently bound to either porous glass or porous silica beads. This immobilized-enzyme system was examined for its use in detoxification of pesticides in production wastewaters. The kinetics of parathion hydrolysis were examined at flow rates up to 96 liter/hr and at influent substrate concentrations ranging from 10--250 mg/liter. The enzyme reactor was able to hydrolyze 95% or more of the parathion added to industrial wastewaters generated during its production, thus reducing the effluent parathion concentration to below 500 ppb. Laboratory continuous-flow experiments were conducted for 70 days with industrial wastewater and indicated no loss in immobilized-enzyme activity. The influence of pH, temperature, solvents, and detergents on enzyme stability and activity and enzyme reactor kinetics will be discussed.

References

Aug 20, 1996·Biotechnology and Bioengineering·K E LeJeune, Alan J Russell
Nov 15, 2002·Biotechnology and Bioengineering·Mark ShimazuWilfred Chen
Nov 15, 1983·Experientia·L M Johnson, H W Talbot
Jan 1, 1996·Applied Biochemistry and Biotechnology·S C Hung, J C Liao
Nov 19, 2013·Journal of Inorganic Biochemistry·Lena J DaumannLawrence R Gahan
Mar 6, 1998·Trends in Biotechnology·W Chen, A Mulchandani
Feb 3, 1999·Annals of the New York Academy of Sciences·K E LeJeuneAlan J Russell
Jul 27, 1999·Chemico-biological Interactions·B D Di SioudiJ R Wild
Mar 3, 2005·Biosensors & Bioelectronics·Brandy J White, H James Harmon
Jul 26, 2005·Biochemical and Biophysical Research Communications·Hong LiuNing-Yi Zhou
Apr 22, 2003·Annual Review of Biomedical Engineering·Alan J RussellRichard R Koepsel
May 12, 2009·Advances in Applied Microbiology·Casey M TheriotAmy M Grunden
Dec 31, 2005·Journal of Bioscience and Bioengineering·Kanako TagoMasahito Hayatsu
May 27, 2011·Journal of Enzyme Inhibition and Medicinal Chemistry·K Y VasanthaA P Sattur

Citations

Jul 1, 1976·Applied and Environmental Microbiology·D M Munnecke
Jul 1, 1972·Biotechnology and Bioengineering·R D Mason, H H Weetall
Mar 1, 1977·Applied and Environmental Microbiology·D M Munnecke

Related Concepts

Phosphoric Monoester Hydrolases
Glass (Substance)
Organopyrophosphorus Compounds
Hydrolysis
Insecticides
Hydrogen-Ion Concentration
Quso G-32

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

© 2020 Meta ULC. All rights reserved