PMID: 2503032Aug 22, 1989Paper

Hydrolysis of triacylglycerol arachidonic and linoleic acid ester bonds by human pancreatic lipase and carboxyl ester lipase

Biochimica Et Biophysica Acta
Q ChenA Nilsson

Abstract

The hydrolysis of polyenoic fatty acid ester bonds with pure human colipase-dependent lipase, with carboxyl ester lipase (CEL) and with these enzymes in combination was studied, using [3H]arachidonic- and [14C]linoleic acid-labelled rat chylomicrons as a model substrate. During the hydrolysis with colipase-dependent lipase, the amount of 3H appearing in 1,2-X-diacylglycerol (DG) markedly exceeded that of 14C. When CEL was added in addition this [3H]DG was efficiently hydrolyzed. CEL alone hydrolyzed the triacylglycerol (TG) at a low rate. The hydrolysis pattern with human duodenal content was similar to that seen with colipase-dependent lipase and CEL in combination. Increasing the concentration of taurodeoxycholate (TDC) and taurocholate (TC) or of TDC alone stimulated the hydrolysis of [3H]- and [14C]TG, but increased the accumulation of labelled DG that could act as substrate for CEL. It is suggested that very-long-chain polyenoic fatty acids of DG formed during the action of the colipase-dependent lipase on TG containing these fatty acids may be a physiological substrate for CEL.

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Citations

Jan 6, 2007·Digestive Diseases and Sciences·David C Whitcomb, Mark E Lowe
Jan 18, 2003·Lipids·Marcel S F Lie Ken JieM L Chye
Dec 1, 1998·Nutrition Research Reviews·J S Forsyth
Apr 1, 1992·Protein Expression and Purification·L P DiPersioD Y Hui
May 6, 2015·Atherosclerosis. Supplements·Claudia Stefanutti, Ulrich Julius
Mar 4, 2014·Critical Reviews in Food Science and Nutrition·Claire BourlieuDidier Dupont
Mar 30, 2017·Journal of the American Chemical Society·Andrew T NelsonDionicio Siegel
Jan 1, 1990·Acta Paediatrica Scandinavica. Supplement·O Hernell
Aug 1, 1991·Scandinavian Journal of Gastroenterology·B SternbyB Borgström
Aug 22, 2001·Biochimica Et Biophysica Acta·D Lombardo
Sep 15, 1995·Journal of Chromatography. B, Biomedical Applications·V Ruiz-Gutiérrez, L J Barron
Mar 4, 1992·Biochimica Et Biophysica Acta·D R GjellesvikB T Walther

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