Hydrolysis of whey lactose using CTAB-permeabilized yeast cells

Bioprocess and Biosystems Engineering
Gurpreet KaurHarish Kumar

Abstract

Disposal of lactose in whey and whey permeates is one of the most significant problems with regard to economics and environmental impact faced by the dairy industries. The enzymatic hydrolysis of whey lactose to glucose and galactose by beta-galactosidase constitutes the basis of the most biotechnological processes currently developed to exploit the sugar content of whey. Keeping this in view, lactose hydrolysis in whey was performed using CTAB permeabilized Kluyveromyces marxianus cells. Permeabilization of K. marxianus cells in relation to beta-galactosidase activity was carried out using cetyltrimethyl ammonium bromide (CTAB) to avoid the problem of enzyme extraction. Different process parameters (biomass load, pH, temperature, and incubation time) were optimized to enhance the lactose hydrolysis in whey. Maximum hydrolysis (90.5%) of whey lactose was observed with 200 mg DW yeast biomass after 90 min of incubation period at optimum pH of 6.5 and temperature of 40 degrees C.

References

Jun 1, 1980·Journal of Bacteriology·R C Dickson, J S Markin
Nov 27, 2002·Biotechnology and Bioengineering·Quinn Zhengkun ZhouXuemei Li
Sep 26, 2003·Journal of Dairy Science·A N GenariF M L Passos
Dec 16, 2003·Applied Microbiology and Biotechnology·Y-J LeeD-K Oh
Aug 27, 2004·Biotechnology and Bioengineering·Ye Ni, Rachel R Chen
May 1, 1994·Biotechnology and Bioengineering·S T YangG Hong

❮ Previous
Next ❯

Citations

Dec 16, 2011·Critical Reviews in Biotechnology·Parmjit S Panesar, John F Kennedy
Mar 29, 2011·International Journal of Biological Macromolecules·Qayyum HusainAmeer Azam
May 29, 2015·Biotechnology, Biotechnological Equipment·Ilona Trawczyńska, Marek Wójcik
Feb 7, 2014·Applied Biochemistry and Biotechnology·Jay Shankar Singh YadavR Y Surampalli
Sep 19, 2009·Journal of Dairy Science·I Moreno-IndiasA Argüello

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.