Hydrolytic enzymes in KB cells infected with poliovirus and herpes simplex virus.

Journal of Bacteriology
J F Flanagan

Abstract

Flanagan, John F. (Duke University School of Medicine, Durham. N.C.). Hydrolytic enzymes in KB cells infected with poliovirus and herpes simplex virus. J. Bacteriol. 91:789-797. 1966.-The effect of poliovirus and herpes simplex virus infection on the activity of five hydrolytic enzymes was studied in tissue culture cells of KB type. During the course of poliovirus infection, the activity of beta-glucuronidase, acid protease, acid ribonuclease, acid deoxyribonuclease, and acid phosphatase in the cytoplasm rose to levels two- to fourfold greater than the activity present in the cytoplasm of uninfected cells. The rise in cytoplasmic activity was accompanied by a concomitant decrease in enzymatic activity bound to cell particles. Shift of enzymatic activity from the particulate to soluble state was first detected at 6 hr after poliovirus infection, coinciding with the appearance of new infectious particles and virus cytopathic effect. No net synthesis of these enzymes after poliovirus infection was found. Hydrocortisone added to the culture medium failed to affect either the titer of virus produced in the cells or the release of hydrolytic enzymes from the particulate state. Herpes simplex infection produced minimal alterations in ...Continue Reading

Citations

May 2, 1974·The New England Journal of Medicine·S M Sabesin, R S Koff
Dec 5, 2012·PLoS Pathogens·Alexsia L Richards, William T Jackson
Feb 1, 1970·Journal of Virology·D L FineE H Ludwig
Sep 1, 1969·Bacteriological Reviews·A Lwoff
Mar 1, 1982·Antimicrobial Agents and Chemotherapy·P GarrityR Adler
Sep 1, 1969·Journal of Virology·K E Blackman, H C Bubel
Nov 1, 1974·Journal of Virology·L E Guskey, D A Wolff
Apr 1, 1971·Experimental and Molecular Pathology·S M SabesinK J Isselbacher
Feb 1, 1968·Experimental Cell Research·H Thacore, D A Wolff

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