Hydrolyzed eggshell membrane immobilized on phosphorylcholine polymer supplies extracellular matrix environment for human dermal fibroblasts.

Cell and Tissue Research
Eri Ohto-FujitaYoriko Atomi

Abstract

We have found that a water-soluble alkaline-digested form of eggshell membrane (ASESM) can provide an extracellular matrix (ECM) environment for human dermal fibroblast cells (HDF) in vitro. Avian eggshell membrane (ESM) has a fibrous-meshwork structure and has long been utilized as a Chinese medicine for recovery from burn injuries and wounds in Asian countries. Therefore, ESM is expected to provide an excellent natural material for biomedical use. However, such applications have been hampered by the insolubility of ESM proteins. We have used a recently developed artificial cell membrane biointerface, 2-methacryloyloxyethyl phosphorylcholine polymer (PMBN) to immobilize ASESM proteins. The surface shows a fibrous structure under the atomic force microscope, and adhesion of HDF to ASESM is ASESM-dose-dependent. Quantitative mRNA analysis has revealed that the expression of type III collagen, matrix metalloproteinase-2, and decorin mRNAs is more than two-fold higher when HDF come into contact with a lower dose ASESM proteins immobilized on PMBN surface. A particle-exclusion assay with fixed erythrocytes has visualized secreted water-binding molecules around the cells. Thus, HDF seems to possess an ECM environment on the newly de...Continue Reading

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Citations

Dec 26, 2013·International Journal of Pharmaceutics·George Dan Mogoşanu, Alexandru Mihai Grumezescu
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Mahesh Kumar Sah, Subha Narayan Rath
Nov 30, 2016·International Journal of Molecular Sciences·Atul A ChaudhariShreekumar R Pillai
Nov 19, 2018·Cell and Tissue Research·Eri Ohto-FujitaYoriko Atomi
Mar 26, 2021·Journal of Biomedical Materials Research. Part a·Armin AmirsadeghiSeyeddeh-Sara Hashemi

Methods Mentioned

BETA
atomic force microscopy
gel-filtration
Assay
PCR
confocal microscopy
size-exclusion chromatography
AFM

Related Concepts

2-methacryloyloxyethyl phosphorylcholine
Alkalies
Metazoa
Aves
Cell Adhesion
Egg Shell
Extracellular Matrix
Fibroblasts
Gene Expression Regulation
Glycosaminoglycans

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