Hydroperoxidative oxidation of diethylstilbestrol by lipoxygenase

Archives of Biochemistry and Biophysics
E Núñez-DelicadoF García-Carmona

Abstract

The oxidation of diethylstilbestrol (DES), a synthetic carcinogenic estrogen, by the hydroperoxidase activity of lipoxygenase was studied. Lipoxygenase catalyzes the oxidation of DES to its corresponding DES quinone to yield free radical species intermediates (DES semiquinone and DES quinone), which are associated with the adverse effects of this synthetic estrogen. The reaction was dependent on enzyme, DES, and hydrogen peroxide concentrations. Due to the low degree of water solubility of DES, the enzyme works in a range of DES concentrations below K(m). The enzyme presents a high affinity for hydrogen peroxide (5.7 microM), and produces substrate inhibition (Ksi = 2.5 mM). This study is the first demonstration that this reaction, which is known to be catalyzed by a variety of enzymes, including peroxidases, is also catalyzed by lipoxygenase.

References

Oct 1, 1977·Journal of Toxicology and Environmental Health·M MetzlerW C Hobson
Dec 14, 1978·Biochemical and Biophysical Research Communications·M Metzler, J A McLachlan
Sep 15, 1988·Biochemical and Biophysical Research Communications·A P Kulkarni, D C Cook
Jan 1, 1981·CRC Critical Reviews in Biochemistry·M Metzler
Dec 15, 1983·Biochemical Pharmacology·J G LiehrJ Henkin
Sep 15, 1994·Biochimica Et Biophysica Acta·M Pérez-GilabertF García-Carmona
Apr 1, 1994·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K A Akhilender NaiduA P Kulkarni
Jun 15, 1994·Biochemical Pharmacology·M Pérez-GilabertF García-Carmona
Apr 15, 1994·Biochemical and Biophysical Research Communications·M A RoseiR Coccia
Mar 7, 1996·Biochimica Et Biophysica Acta·E Núñez-DelicadoF García-Carmona
Jun 4, 1962·Biochimica Et Biophysica Acta·H J EICHEL, W W WAINIO

❮ Previous
Next ❯

Citations

Jun 20, 2002·Pharmacology & Toxicology·Toshiaki MiuraYukio Fujimoto
Mar 25, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Wen G JiangRobert E Mansel
Nov 29, 2005·Journal of Bioscience and Bioengineering·Rizette Avila-GonzálezFrancisco García-Carmona
Jul 9, 1999·Archives of Biochemistry and Biophysics·E Núñez-DelicadoF García-Carmona
Dec 14, 2002·The International Journal of Biochemistry & Cell Biology·Estrella Núñez-DelicadoAlvaro Sánchez-Ferrer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.