Hydroperoxide and carboxyl groups preferential location in oxidized biomembranes experimentally determined by small angle X-ray scattering: Implications in membrane structure

Biochimica Et Biophysica Acta. Biomembranes
Raffaela De RosaRosangela Itri

Abstract

We report small angle X-ray scattering (SAXS) data from large unilamellar vesicles as model membranes composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocoline (POPC) and two oxidized species, namely its hydroperoxidized form POPC-OOH and 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PazePC) lipid that has a carboxyl group at the end of its truncated sn-2 chain. The replacement of POPC by either POPC-OOH (POPC-OOHxPOPC1-x) or PazePC (PazePCxPOPC1-x), with oxidized lipid molar ratio x varying from 0.00 up to 1.00, permits to experimentally inspect changes in the membrane structural properties due to oxidation. The volume fraction distribution of each lipid chemical group along the bilayer is determined. The results quantify that 95% of the hydroperoxide group lies in the membrane polar moiety, near the carbonyl and phosphate groups, whereas just 5% of OOH group experiences the polar/apolar interface, for all values of x studied. In the case of PazePC up to x = 0.33, a bimodal distribution of the carboxyl group in the interior and polar regions of the lipid membrane is obtained, probably due to a dynamic movement of the shortened alkyl chain towards the water interface. The mean molecular area A gradually increases from 6...Continue Reading

Citations

May 10, 2019·Journal of Leukocyte Biology·Yulia Y TyurinaValerian E Kagan
Feb 20, 2020·Journal of Biomolecular Structure & Dynamics·Surendra KumarDharmendra K Yadav
Sep 22, 2020·Biophysics Reviews·Rosangela Itri
Aug 14, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Francesco SpinozziPaolo Mariani
Jul 10, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Natalia A CorvalánRamiro Lascano
Nov 8, 2020·Archives of Biochemistry and Biophysics·Tayana Mazin TsuboneMauricio S Baptista
Jan 9, 2021·Journal of Cell Science·Kazuhiro KajiwaraYoel Sadovsky
Jun 4, 2021·Journal of Chemical Information and Modeling·Maria C OliveiraRodrigo M Cordeiro
May 18, 2021·Free Radical Biology & Medicine·Julia Bahja, Marcus K Dymond
May 31, 2021·Biochimica Et Biophysica Acta. Biomembranes·Helena JunqueiraCarlos M Marques
Aug 11, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Gustavo ScanavachiRosangela Itri
Aug 11, 2021·Journal of Cell Science·Kazuhiro KajiwaraYoel Sadovsky
Jan 1, 2020·ACS Omega·Isabel O L Bacellar, Mauricio S Baptista
Sep 30, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Florian GellertChristiane A Helm
Sep 4, 2021·Biophysical Journal·Phansiri BoonnoyJirasak Wong-Ekkabut

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