Hydrophilic interaction chromatography of small metal species in plants using sulfobetaine- and phosphorylcholine-type zwitterionic stationary phases

Journal of Separation Science
Günther WeberHeiko Hayen

Abstract

Hydrophilic interaction chromatography (HILIC) was applied for the separation of small, noncovalent metal species, and free ligands in plants, using two different zwitterionic stationary phases at pH 7.3. Ligands ranged from amino acids and phytosiderophores to organic acids and synthetic chelators like EDTA. Our results confirmed the suitability of zwitterionic stationary phases for the separation of such ligands and their metal complexes. In particular, the chromatographic behavior of phytosiderophore complexes of copper, nickel, and zinc on a sulfobetaine-type material was compared to that on a phosphorylcholine-type material under otherwise identical conditions. A compression of the usable retention range for phytosiderophore species on the latter phase was found, which can be traced back to the reversed charge orientation of the zwitterionic functionalities. Differences were also found for the integrity of more labile metal chelates during separation on the two columns. In particular, Ni-malate could only be analyzed on the sulfobetaine phase, and Cu-glutathione, Ni-aspartate, and Ni-citrate complexes were only stable on the phosphorylcholine stationary phase at pH 7.3. Ni-histidine species were only found after lowering t...Continue Reading

References

Aug 19, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·In Bok PaekHye Suk Lee
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Jan 25, 2005·Plant Physiology·Dominique LoquéNicolaus von Wirén
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Sep 5, 2007·Electrophoresis·Yue XuanGünther Weber

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Citations

Mar 16, 2013·Journal of Chromatographic Science·Giorgia Greco, Thomas Letzel
Feb 15, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tomoko ArigaEtsuro Yoshimura
Nov 3, 2016·Journal of Separation Science·David Matějíček, Jana Vašíčková
Sep 30, 2009·Analytica Chimica Acta·Ekaterina P NesterenkoBrett Paull
May 8, 2009·Chemical Society Reviews·Sandra MounicouRyszard Lobinski
Jul 17, 2009·Journal of Separation Science·Zhengjin JiangMark R Taylor
Mar 9, 2010·Electrophoresis·Madeleine Dell'mourStephan Hann
Jun 30, 2018·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Karen ScholzHeiko Hayen
Jun 21, 2017·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Matthias BauneHeiko Hayen
Jan 16, 2021·International Journal of Molecular Sciences·Tomasz K GozdziewiczJolanta Lukasiewicz
Jul 22, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yusuke IwasakiHiroyuki Nakazawa

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