Hydrophilic interaction chromatography/tandem mass spectrometry for the determination of melamine in royal jelly and royal jelly lyophilized powder

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Jinhui ZhouJianqiu Mi

Abstract

Melamine has become the focus of attention for the possible occurrence of nephrolithiasis and associated deaths, because it was added to foods to increase the apparent protein content by unethical manufacturers. An analytical method based on hydrophilic interaction chromatography/tandem mass spectrometry (HILIC-MS/MS) was developed and validated for the determination of melamine in the royal jelly (RJ) and royal jelly lyophilized powder (RJLP). Trace of melamine was extracted from the RJ and RJLP by ultrasonic-assisted extraction followed by clean-up procedure using mixed-mode cation exchange (MCX) solid phase extraction and separated on a hydrophilic interaction chromatography (HILIC) analytical column with acetonitrile/5mM ammonium acetate buffer (88:12, v/v) as mobile phase. Detection was carried out by positive electrospray ionization (ESI+) in multiple reaction monitoring (MRM) mode. The chromatographic separation was obtained within 5min and was linear in the concentration range of 0.01-8microg/mL in RJ and 0.05-10microg/mL in RJLP for melamine. The mean extraction recoveries for melamine were ranged from 89.6 to 100.4%. Method validation parameters were evaluated such as linearity, selectivity, precision, carryover and r...Continue Reading

References

Aug 24, 2000·Journal of Agricultural and Food Chemistry·R A YokleyM W Cheung
Nov 15, 2007·Rapid Communications in Mass Spectrometry : RCM·Michael S FiligenziBirgit Puschner
Jul 26, 2008·Journal of Agricultural and Food Chemistry·Michael S FiligenziRobert H Poppenga
Oct 31, 2008·Lancet·E Y Y ChanC W Chan
May 14, 2009·Rapid Communications in Mass Spectrometry : RCM·Wei-Chih ChengDaniel Yang-Chih Shih

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