Hydrophilic interaction LC of peptides: columns comparison and clustering

Journal of Separation Science
Sylvia Van DorpeBart De Spiegeleer

Abstract

A wide variety of hydrophilic interaction chromatography (HILIC) stationary phase surface chemistries are currently available. Although their selectivity can be considerably different, column comparison or clustering using peptides is limited. In this study, ten pharmaceutically relevant model peptides are analyzed on seven different HILIC columns (bare silica, amide, poly-hydroxyethyl aspartamide, diol and zwitterionic) for the evaluation of their performance and classification. The responses examined include single and multiple responses: plate number, asymmetry factor, LOD, geometric mean resolution, resolution product, time corrected resolution product, peak capacity and chromatographic response function. Column classification was performed using hierarchical clustering and principal component analysis. Moreover, the overall performance quality of the HILIC columns was compared using a linear desirability function. Hierarchical clustering and principal component analysis showed consistent clusters. The zwitterionic phase was clustered apart from the other HILIC columns and both poly-aspartamide columns were clustered together. In addition, the two bare silica phases represent two different clusters, and thus different selec...Continue Reading

References

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Jan 20, 2009·Journal of Pharmaceutical and Biomedical Analysis·Adel PezeshkiBart De Spiegeleer

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Citations

May 1, 2012·Analytical and Bioanalytical Chemistry·Christelle Harscoat-SchiavoIvan Marc
Mar 19, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anja EkdahlMartin Ahnoff
Feb 6, 2014·PloS One·Anton De SpiegeleerDirk Elewaut
Feb 5, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Sylvia Van DorpeBart De Spiegeleer
Aug 9, 2016·Journal of Pharmaceutical Sciences·Suzanne M D'AddioAllen C Templeton
Oct 15, 2011·Journal of Separation Science·Maarit KaronenJari Sinkkonen
Dec 29, 2020·Journal of Chromatography. a·Dmitry V YaroshenkoAlla V Chernobrovkina
Aug 2, 2011·Journal of Chromatography. a·Ngoc Phuoc DinhKnut Irgum

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