Hydrophilic interaction liquid chromatography and accurate mass measurement for quantification and confirmation of morphine, codeine and their glucuronide conjugates in human urine

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Marjo KolmonenIlkka Ojanperä

Abstract

A hydrophilic interaction liquid chromatography-time-of-flight mass spectrometry (HILIC-TOFMS) method for the quantification and confirmation of morphine (M), codeine (C), morphine-3-glucuronide (M3G), morphine-6-glucuronide (M6G) and codeine-6-glucuronide (C6G) is presented. The method was validated in terms of specificity, selectivity, extraction recovery, accuracy, repeatability, linearity and matrix effect. After a straightforward sample preparation by solid phase extraction (SPE) the compounds were analyzed directly without the need for hydrolysis, solvent transfer, evaporation or reconstitution. The HILIC technique provided good chromatographic separation which was critical for isomers M3G and M6G. The analytes were detected after electrospray ionization (ESI) in positive mode with mass accuracies below 2 mDa using a 5-mDa window. A measurement range of 50-5000 ng/ml was applied for calibration using deuterated analogs as internal standards. The precision of the method was 5.7% and 10.2% (RSD) within and between days, respectively. The applicability of the method was demonstrated with authentic urine samples known to contain codeine and/or morphine and their intact glucuronide conjugates. Identification of the analytes wa...Continue Reading

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Citations

Jun 18, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·M M Delgado-ZamarreñoR Carabias-Martínez
Jan 15, 2014·Life Sciences·Mahmoud A ElsohlyMohamed M Radwan
Mar 24, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Eman G NoumanHayam M Lotfy
Jan 29, 2016·Journal of Chromatography. a·Stéphanie RomandDavy Guillarme
Aug 10, 2011·Forensic Science International : Synergy·Flavia BadoudJean-Luc Veuthey
Oct 25, 2013·Proteomics·Roman FischerBenedikt M Kessler
Jul 22, 2014·Bioanalysis·Shanlin FuUnnikrishnan Kuzhiumparambil
Jun 28, 2018·Environmental Science and Pollution Research International·Meena K YadavChristopher P Saint
Jul 7, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Soledad González-RubioSoledad Rubio
May 3, 2011·Analytical Chemistry·T A BrettellJ R Almirall

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