Hydrophilic interaction ultra performance liquid chromatography retention prediction under gradient elution

Analytical and Bioanalytical Chemistry
Helen G GikaA Pappa-Louisi

Abstract

The development and application of new separation mechanisms such as hydrophilic interaction chromatography (HILIC) is of high importance for the simultaneous analysis of polar molecules such as primary metabolites. However the retention mechanism in HILIC is not fully understood and as a result retention prediction tools are not at hand for this chromatographic approach. In the present report we study the utility of a simple algorithm, based on a simple linear and/or a simple logarithmic retention model, for retention prediction in HILIC gradient separation of a mixture of 23 selected compounds including (poly)amines, amino acids, saccharides, and other molecules. Utilizing two types of gradient elution programs with or without an isocratic part, retention data were collected in order to build prediction models. Starting from at least three gradient runs the prediction of analyte retention was very satisfactory for all gradient programs tested, providing useful evidence of the value of such retention time prediction methodologies.

References

Nov 15, 2008·Molecular & Cellular Proteomics : MCP·Kyoungmi KimRobert H Weiss
Aug 12, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Megumi KatoAkiko Takatsu
Feb 27, 2010·Journal of Separation Science·Konstantina SpagouGeorgios Theodoridis
Mar 12, 2010·Journal of Separation Science·Giorgio MarrubiniGabriella Massolini
Dec 15, 2010·Analytical Chemistry·Konstantina SpagouElizabeth J Want
Jan 1, 2010·Bioinformation·Takashi HagiwaraTatsunari Nishi
Mar 9, 2011·Mass Spectrometry Reviews·Georgios TheodoridisIan D Wilson

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Citations

Mar 13, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Helen G GikaGeorgios A Theodoridis
Mar 16, 2013·Journal of Chromatographic Science·Giorgia Greco, Thomas Letzel
Dec 12, 2012·Analytical and Bioanalytical Chemistry·Ron WehrensStefan Martens
Oct 25, 2013·Proteomics·Roman FischerBenedikt M Kessler
Feb 26, 2020·Journal of Separation Science·Tijmen S BosBob W J Pirok
Jan 31, 2021·Metabolites·Georgios TheodoridisOn Behalf Of The FoodOmicsGR Ri Consortium
Mar 4, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xingchen ZhaiJing Wang

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