Hydrophilic Nanocomposite Functionalized by Carrageenan for the Specific Enrichment of Glycopeptides

Analytical Chemistry
Yingxin ChenMinbo Lan

Abstract

A hydrophilic nanocomposite was synthesized by an easy route to improve glycopeptides enrichment efficiency. This new composite, prepared with a method based on electrostatic interaction, was demonstrated to be efficient for immobilization of carrageenan on graphene oxide/poly(ethylenimine) support (denoted as GO-PEI-Carr). Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics. The introduction of carrageenan provided the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides. Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio. A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500. Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biologi...Continue Reading

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Citations

Aug 16, 2019·Chemical Communications : Chem Comm·Nianrong SunChunhui Deng
Oct 28, 2019·Rapid Communications in Mass Spectrometry : RCM·Bin LiuChuanfan Ding
Feb 7, 2020·Analytical and Bioanalytical Chemistry·Huihui WanXinmiao Liang
Jan 14, 2021·Carbohydrate Polymers·Pravin Shende, Nazneen Pathan
Apr 11, 2021·International Journal of Biological Macromolecules·Yu-Qiao WangHai-Tao Wu
Sep 14, 2021·Analytical Methods : Advancing Methods and Applications·Zheng GaoJunjie Ou

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