Hydrophilic probe in mesoporous pore for selective enrichment of endogenous glycopeptides in biological samples

Analytica Chimica Acta
Yilin LiNianrong Sun

Abstract

As one of the most important post-translational modifications (PTMs) of peptidome, glycopeptidome is closely related to serious disease, especially to cancer. In order to specifically discover and analyze glycopeptidome biomarkers for clinical diagnosis of cancer on early-stage, it is crucial to develop efficient technique to analyze low-abundance of endogenous glycopeptides in biological samples. In this report, a hydrophilic probe in mesoporous pore (denoted as Fe3O4@mSiO2@G6P) was designed and prepared. By taking advantage of the excellent hydrophilicity and size-exclusion ability, we applied Fe3O4@mSiO2@G6P to capture glycopeptides from both horseradish peroxidase (HRP) and immunoglobulin (IgG) digests successfully. Moreover, a total of 39 and 25 endogenous glycopeptides were identified from healthy saliva and gastric saliva, respectively, indicating the great potential of this probe for the exploration of glycopeptidome biomarkers.

Citations

Aug 16, 2019·Chemical Communications : Chem Comm·Nianrong SunChunhui Deng
Oct 28, 2019·Rapid Communications in Mass Spectrometry : RCM·Bin LiuChuanfan Ding

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