Hydrophobic chromatography of cells: adsorption and resolution on homologous series of alkylagaroses

Journal of Chromatography
G HalperinS Shaltiel

Abstract

Implantation of hydrocarbon chains on beaded agarose results in column materials capable of adsorbing erythrocytes or lymphocytes from various sources. In a homologous series of such columns, identical in all structural respects (ligand density, charge density and ultrastructure) except for the length of their hydrocarbon chains, the capacity to adsorb these cells generally increases with increase in the number of carbon atoms per chain. The cells can be desorbed from the columns by repeated gentle pipetting in the presence of bovine serum albumin (for erythrocytes) or foetal calf serum (for lymphocytes). Under the conditions used for adsorption and desorption there is neither physical entrapment of the cells in the column nor apparent damage to their integrity, as indicated by the facts that (a) 95-98% of the cells applied on the column can be recovered; (b) erythrocytes eluted from the column and those yet to be applied are morphologically indistinguishable, exhibit an identical osmotic fragility profile and, (after desorption) retain the same adsorption profile for the columns; and (c) over 95% of the mouse spleen lymphocytes, eluted from the columns, continue to exclude the dye Trypan blue, suggesting that they preserve the...Continue Reading

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Citations

May 24, 1991·Biochimica Et Biophysica Acta·H G BotrosM A Vijayalakshmi
Mar 20, 2014·Journal of Cellular Biochemistry·Daniel HagenfeldPeter Prehm
Sep 14, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Raha PourbaghiReihaneh Shamsfar
Apr 1, 1995·Transfusion Medicine Reviews·A BruilW G van Aken

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