Hydrophobic high-performance liquid chromatography of hormonal polypeptides and proteins on alkylsilane-bonded silica

Journal of Chromatography
M J O'Hare, E C Nice


Thirty-two hormonal polypeptides and nine proteins (8-65 kD) have been used to evaluate the potential of high-performance liquid chromatography on alkylsilane-bonded silica for separating and recovering biologically active compounds of this type. The basic method used was gradient elution with acetonitrile in an acid phosphate buffer. Variation of key chromatographic parameters demonstrated that low pH (less than 4.0) and high buffer molarity (greater than 0.1 M) are mandatory for reproducible high efficiency polypeptide chromatography. Simple NaCl-HCl mixtures of appropriate acidity and molarity could be substituted for the acid phosphate buffer, with the advantage of minimising non-physiological ion contributions to eluted materials. Minor selective effects were noted with different organic modifiers, but variation of other parameters, including choice of specific alkylsilane packings, did not materially influence separations. Under optimal conditions all of the polypeptides tested could be efficiently chromatographed, and many simultaneously resolved, as could most of the proteins tested. Three of the more hydrophobic proteins could not, however, be eluted from the alkylsilane packings. Retention orders of smaller compounds ...Continue Reading


Sep 26, 2002·Electrophoresis·Yufeng Shen, Richard D Smith
Aug 1, 1980·Pflügers Archiv : European journal of physiology·F T LaRochelleP Stern
Mar 1, 1986·American Journal of Obstetrics and Gynecology·Z BlumenfeldR B Jaffe
Mar 29, 1996·Journal of Chromatography. a·S YamakiT Shinoda
Jan 1, 1989·Journal of Pharmaceutical and Biomedical Analysis·E C Nice, R J Simpson
Feb 25, 1983·Journal of Chromatography·B S WelinderJ S Brush
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J L Meek
Jan 1, 1984·Preparative Biochemistry·H J CornellA C Herington
Jul 1, 1986·The Journal of Clinical Investigation·Z Blumenfeld, R B Jaffe
Oct 1, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Noriyuki NimuraHiroshi Homma
Apr 27, 2013·The Behavioral and Brain Sciences·Nicolas J Bullot, Rolf Reber
Mar 20, 2013·The Behavioral and Brain Sciences·Nicolas J Bullot, Rolf Reber
Mar 15, 1980·Analytical Biochemistry·F E Regnier, K M Gooding
Mar 1, 1984·Analytical Biochemistry·J L FausnaughF E Regnier
Jan 25, 2008·Proteomics·Kosaku ShinodaYasushi Ishihama
Apr 1, 1989·International Journal of Peptide and Protein Research·C G FieldsT A Cross


Nov 12, 1977·British Medical Journal·B I Rees, G Jacob
May 26, 1972·Biochemical and Biophysical Research Communications·L T Hunt, M O Dayhoff

Related Concepts

Phosphate buffers
Gene Products, Protein
Infundibular Stem
Dall Sheep
Tissue Extracts
Posterior Pituitary Hormones
High Pressure Liquid Chromatography Procedure
Salmo salmo

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.